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Temporal dynamics and metagenomics of phosphorothioate epigenomes in the human gut microbiome

ABSTRACT: Epigenetic regulation of gene expression and host defense is well established in microbial communities, with dozens of DNA modifications comprising the epigenomes of prokaryotes and bacteriophage. Phosphorothioation (PT) of DNA, in which a chemically-reactive sulfur atom replaces a non-bridging oxygen in the sugar-phosphate backbone, is catalyzed by dnd and ssp gene families widespread in bacteria and archaea. However, little is known about the role of PTs or other microbial epigenetic modifications in the human microbiome. Here we optimized and applied fecal DNA extraction, mass spectrometric, and metagenomics technologies to characterize the landscape and temporal dynamics of gut microbes possessing PT modifications.

INSTRUMENT(S): 6490 Triple Quadrupole LC/MS, Q Exactive

ORGANISM(S): Escherichia Coli

TISSUE(S): Feces

SUBMITTER: Michael DeMott

LAB HEAD: Peter C. Dedon

PROVIDER: PXD051389 | Pride | 2025-03-03

REPOSITORIES: Pride

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			Action	DRS
	Figure_2A-PTs_in_mouse_gut.zip	Other
	Figure_2B-Bar_Plot_of_PTs_in_11_Donors.zip	Other
	Figure_2C-Time_Course_for_Donor5.zip	Other
	Figure_2D-m6dA_for_Donor5.zip	Other
	Figure_S2-HRMS_Confirmation_of_PT_Dinucleotides.zip	Other

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