Dataset Information

Response of C. jejuni to various environmental conditions

ABSTRACT: Campylobacter jejuni is the leading cause of foodborne human gastroenteritis in the developed world. Infections are largely acquired from poultry produced for human consumption and poor food handling is thus a major risk factor. In this study, C. jejuni were exposed to growth in a number of enviornmental conditions representative of the human gastrointestinal tract, including 0.1% deoxycholate (DOC), under iron limitation (induced by 1 mM deferroxamine, in the presence of chicken 'juice' or 'exudate (the thaw water of frozen commerical chicken products) and in the presence of mammalian mucin.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Campylobacter Jejuni Subsp. Jejuni Nctc 11168 = Atcc 700819 Bacteria

SUBMITTER: Melanie White

LAB HEAD: Stuart Cordwell

PROVIDER: PXD051724 | Pride | 2024-06-23

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20180712_QEHF_LM_O_DOC_deferro_exu_f-2.2.0.388.mzML	Mzml
	20180712_QEHF_LM_O_DOC_deferro_exu_f-2.2.0.388.mzid.gz	Mzid
	20180712_QEHF_LM_O_DOC_deferro_exu_f-2.2.0.388.pdResult	Other
	20180712_QEHF_LM_O_DOC_deferro_exu_f-2.2.0.388.pdResultView	Other
	20180712_QEHF_LM_O_DOC_deferro_exu_f1.raw	Raw

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Publications

Multi-Omics of Campylobacter jejuni Growth in Chicken Exudate Reveals Molecular Remodelling Associated with Altered Virulence and Survival Phenotypes.

Man Lok L Soh Pamela X Y PXY McEnearney Tess E TE Cain Joel A JA Dale Ashleigh L AL Cordwell Stuart J SJ

Microorganisms 20240425 5

Campylobacter jejuni is the leading cause of foodborne human gastroenteritis in the developed world. Infections are largely acquired from poultry produced for human consumption and poor food handling is thus a major risk factor. Chicken exudate (CE) is a liquid produced from defrosted commercial chicken products that facilitates C. jejuni growth. We examined the response of C. jejuni to growth in CE using a multi-omics approach. Changes in the C. jejuni proteome were ...[more]

PMID: 38792690

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Project description:Campylobacter jejuni (C. jejuni) is a zoonotic pathogen that causes human diarrhea worldwide. Chickens are a natural reservoir of C. jejuni. Understanding the host response to C. jejuni infection at the molecular level will lay the foundation to control human campylobacterosis by reducing food contamination. Two distinct genetic lines, resistant (line A) and susceptible (line B) to C. jejuni colonization, were utilized to profile the host response to C. jejuni infection using an Agilent chicken 44K microarray. Day-old chickens were challenged orally with C. jejuni and spleens collected for total RNA 7 days post-challenge. Twenty infected samples with highest (a) or lowest bacterial number (b) in cecal content and twenty non-infected (c) in each line were randomly pooled into four biological replicates. The pair comparisons among these three groups within each line were analyzed. The signal intensity of each gene was normalized using LOWESS method. A mixed model was used to identify differentially expressed genes by SAS (P < 0.001). This was opposite to previous cecal tonsil microarray result. There were 468, 743, and 939 genes differentially expressed between groups a and c, groups a and b, and groups b and c in line A, respectively, and 201, 37, 126 genes in line B, respectively. More differentially expressed genes in spleen in line A than in line B were found. The results indicated that significantly different response to C. jejuni infection occurred between resistant and susceptible chicken lines, and the effects of interaction between genetics and tissue should be considered. Chickens in two broiler lines were inoculated with 10^5 cfu C. jejuni on one day after hatch. The cecal content and cecal tonsil was collected and bacterial number in cecal content was counted on day 7 after inoculation. Twenty samples were separated into 3 groups (high burden, low burden, and control) based on bacterial burden of cecal content in each line, 5 samples were mixed randomly into one pool. A dual color, balanced design was carried on for all samples. Three comparisons were used in each line, non-infected/susceptible, susceptible/resistant, resistant/non-infected, totally, four biological replicates in each line. A Dye swap was used in each pair of comparisons including AN/AS, AS/AR, AR/AN; BN/BS, BS/BR, and BR/BN. Background subtracted signal intensity were collected from 24 arrays and normalized for data analysis.

Dataset Information

Response of C. jejuni to various environmental conditions

Dataset's files

Publications

Multi-Omics of <i>Campylobacter jejuni</i> Growth in Chicken Exudate Reveals Molecular Remodelling Associated with Altered Virulence and Survival Phenotypes.

Similar Datasets

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