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Nanobodies as novel tools to monitor the mitochondrial fission factor Drp1

ABSTRACT: In cells, mitochondria undergo constant fusion and fission. An essential factor for fission is the mammalian dynamin-related protein 1 (Drp1). Dysregulation of Drp1 has been linked to neurodegenerative diseases including Parkinson’s as well as cardiovascular diseases and cancer. Here, we developed nanobodies (Nbs) for proteomics, advanced microscopy and live cell imaging of Drp1. To specifically enrich endogenous Drp1 with interacting proteins for proteomics, we functionalized high-affinity Nbs as capture matrices. Considering the wide range of research applications, the presented Nb toolset will open up new possibilities for advanced functional studies of Drp1 in disease-relevant models.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Claudia Cavarischia Rega

LAB HEAD: Ulrich Rothbauer

PROVIDER: PXD051737 | Pride | 2024-05-29

REPOSITORIES: Pride

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			Action	DRS
	20220625_CCR_A030_Rep1_13_D63.raw	Raw
	20220625_CCR_A030_Rep1_1_Crtl_nhs.raw	Raw
	20220625_CCR_A030_Rep1_7_D7.raw	Raw
	20220626_CCR_A030_Rep2_14_D63.raw	Raw
	20220626_CCR_A030_Rep2_2_Crtl_nhs.raw	Raw

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