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Persistent epithelial barrier dysfunction and Periostin accumulation in histologic remission Eosinophilic Esophagitis

ABSTRACT: Patients with Eosinophilic esophagitis (EoE) require long-lasting resolution of inflammation to prevent fibrostenosis and dysphagia. However, the dissociation between symptoms and histologic improvement suggests persistent molecular drivers despite remission. We aimed to characterize persisting molecular alterations in pediatric patients with EoE using tissue proteomics. Esophageal tissue biopsies (n=151) and clinical data were collected prospectively from pediatric patients with EoE (N=34), gastroesophageal reflux disease (GERD; N=10; inflammatory controls) or functional disorders (FD; N=20; non-inflammatory controls). Biospies (n=131) acquired from the diagnostic endoscopy and up to 7 follow-ups were considered for proteome analysis in patients with EoE, while for GERD and FD only biopsies from initial diagnosis were included. Histologically active EoE (15 eosinophils per high power field (hpf)) was diagnosed in 79 biopsies and 52 samples derived from patients with EoE in remission (15 eosinophils per hpf). After microdissection of the epithelium proteins were extracted from the esophageal tissue followed by a liquid chromatography-tandem mass spectrometry. Proteomic analysis identified 3,704 different proteins in total across all samples.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Esophagus

DISEASE(S): Eosinophilic Esophagitis

SUBMITTER: Ignasi Forne

LAB HEAD: Tobias Schwerd, MD

PROVIDER: PXD052250 | Pride | 2024-09-23

REPOSITORIES: Pride

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			Action	DRS
	Groups_Ref6531_Ref6780_ALL_20220202.txt	Txt
	Ref6531_MLF_RC1_20211119.raw	Raw
	Ref6531_MLF_RC2_20211119.raw	Raw
	Ref6531_MLF_RC3_20211119.raw	Raw
	Ref6531_MLF_RC4_20211119.raw	Raw

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Persistent epithelial barrier dysfunction and Periostin accumulation in histologic remission Eosinophilic Esophagitis

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