Proteomics

Dataset Information

0

Development of an Automated, Ultra-Rapid Bottom-Up Proteomics Workflow Utilizing Alginate-Based Hydrogels


ABSTRACT: A new approach to sample preparation and enzymatic digestion in bottom-up proteomics has been developed using alginate-based hydrogel entrapment of enzymes. This hydrogel facilitates rapid and room-temperature digestions with multienzyme capabilities. Three methodologies were tested: within microcentrifuge tubes, in situ pipette tips, and automated robotic liquid handling. Factorial experimental design identified a 1 h, room temperature, pepsin–trypsin dual-enzyme digestion as optimal for sequence coverage and protein group identification, comparable to a gold-standard overnight proteomic protocol. This method promises significant advancements in proteomic analysis by enhancing reusability, speed, throughput, convenience, and cost-effectiveness, without hindering digestion efficiency.

INSTRUMENT(S): timsTOF SCP

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Maor Arad  

LAB HEAD: Leonard J.

PROVIDER: PXD052369 | Pride | 2024-11-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
checksum.txt Txt
fasta.zip Other
raw.zip Other
searchtype.zip Other
Items per page:
1 - 4 of 4

Similar Datasets

2019-11-12 | PXD010960 | Pride
2024-06-10 | PXD051564 | Pride
2023-08-15 | PXD042696 | Pride
2024-05-29 | PXD052497 | Pride
2024-09-25 | PXD052964 | Pride
2024-07-03 | PXD048638 | Pride
2023-07-11 | PXD041207 | Pride
2019-05-06 | PXD012911 | Pride
2019-05-06 | PXD012928 | Pride
2018-04-18 | PXD004504 | Pride