Proteomics

Dataset Information

0

A photo-caged cross-linker for identifying protein-protein interactions


ABSTRACT: In recent years cross-linking mass spectrometry (XL-MS) as a tool to study protein-protein interactions (PPIs) has undergone significant improvements, which were mainly propelled by the emergence of novel crosslinkers and the development and implementation of advanced analysis workflows. Despite these recent advancements, addressing PPIs in the cell by XL-MS remains a substantial challenge. Some of the current limitations are linked to a comparable poor membrane permeability of most crosslinkers and their limited efficiency to traverse the cellular membrane. To address this problem, we have designed and synthesized a novel crosslinker based on the chemical o-nitrobenzylester (o-NBE) group, which enhances the hydrophobic properties of the cross-linker.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Serum

SUBMITTER: Florian Stengel  

LAB HEAD: Florain Stengel

PROVIDER: PXD052552 | Pride | 2024-11-21

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BSA.fasta Fasta
BSA_BS3.pdAnalysis Other
F2203_chenxi_086.raw Raw
F2203_chenxi_087.raw Raw
F2203_chenxi_088.raw Raw
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