Dataset Information

Bacterial EVs as intranasal postbiotics: Detailed characterization and interaction with airway cells

ABSTRACT: Escherichia coli A0 34/86 (EcO83) is a probiotic bacterium used in newborns to prevent nosocomial infections and diarrhoea. Mechanistically, EcO83 induces the production of pro- and anti-inflammatory cytokines in vitro and in vivo and, when administered intranasally, inhibits allergic airway inflammation in mice by interacting with innate pattern recognition receptors. Despite the proven therapeutic benefit, there is a concern about the use of live bacteria due to the risk of systemic infections and bacterial gene transfer. Therefore, EcO83-derived extracellular vesicles (EcO83-EVs) could represent a safer alternative to live bacteria. However, the exact mechanism of interaction between EV and host remains to be elucidated. Here, we have isolated, purified, and characterised EcO83-EVs following the Minimal Information of Studies of Extracellular Vesicles (MISEV) guidelines. Our ex vivo studies in human nasal epithelial cells showed that EcO83-EVs increased the expression of proteins linked to oxidative stress and inflammation, indicating an effective interaction between EVs and the host cells. Further in vivo studies in mice demonstrated that EcO83-EVs interact with nasal-associated lymphoid tissue, are internalised by airway macrophages, and stimulate neutrophil recruitment in the lung. In vitro analyses revealed that EcO83-EVs activate the NF-κΒ signalling pathway, resulting in the nitric oxide production. Our findings suggest that EcO83-EVs could be an effective and safer postbiotic alternative to live bacteria. Further research is needed to explore the potential of EVs from probiotic bacteria for clinical applications, particularly for mucosal vaccines or targeted immunotherapies.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Akos Vegvari

LAB HEAD: Akos Vegvari

PROVIDER: PXD052553 | Pride | 2024-11-06

REPOSITORIES: pride

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	20200302_HF2_01_Wisgrill_Exp3_1_F1.raw	Raw
	20200302_HF2_02_Wisgrill_Exp3_1_F2.raw	Raw
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	20200302_HF2_04_Wisgrill_Exp3_1_F4.raw	Raw
	20200302_HF2_05_Wisgrill_Exp3_1_F5.raw	Raw

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Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells.

Razim Agnieszka A Zabłocka Agnieszka A Schmid Anna A Thaler Michael M Černý Viktor V Weinmayer Tamara T Whitehead Bradley B Martens Anke A Skalska Magdalena M Morandi Mattia M Schmidt Katy K Wysmołek Magdalena E ME Végvári Akos A Srutkova Dagmar D Schwarzer Martin M Neuninger Lukas L Nejsum Peter P Hrdý Jiri J Palmfeldt Johan J Brucale Marco M Valle Francesco F Górska Sabina S Wisgrill Lukas L Inic-Kanada Aleksandra A Wiedermann Ursula U Schabussova Irma I

Journal of extracellular vesicles 20241001 10

Escherichia coli A0 34/86 (EcO83) is a probiotic strain used in newborns to prevent nosocomial infections and diarrhoea. This bacterium stimulates both pro- and anti-inflammatory cytokine production and its intranasal administration reduces allergic airway inflammation in mice. Despite its benefits, there are concerns about the use of live probiotic bacteria due to potential systemic infections and gene transfer. Extracellular vesicles (EVs) derived from EcO83 (EcO83-EVs) might offer a safer alt ...[more]

PMID: 39429019

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