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Exploring Top-Down Mass Spectrometric Approaches To Probe Forest Cobra (Naja melanoleuca) Venom Proteoforms

ABSTRACT: Three main top-down mass spectrometry methodologies were employed in a proof-of-concept study to characterise selected three finger toxins and phospholipase A2 toxins from the forest cobra (Naja melanoleuca) venom. The novel utility of data-independent acquisition, alternative hybrid ECD-CID fragmentation and the incorporation of cyclic ion mobility separation in a top-down proteomic workflow were explored for snake venom proteins.

INSTRUMENT(S): SELECT SERIES Cyclic IMS, Xevo G2-XS Tof

ORGANISM(S): Naja Melanoleuca

SUBMITTER: Ruth Wang

LAB HEAD: Tara Pukala

PROVIDER: PXD052561 | Pride | 2024-09-05

REPOSITORIES: Pride

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			Action	DRS
	20230920_NM4_cyclic_13.35kDa_mz1669_ECD-CID.raw.zip	Raw
	20230920_NM4_cyclic_13.35kDa_mz1669_ECD-CID_fragment-list.xlsx	Xlsx
	20230920_NM4_cyclic_7.12kDa_mz1187_ECD-CID.raw.zip	Raw
	20230920_NM4_cyclic_7.12kDa_mz1187_ECD-CID_fragment-list.xlsx	Xlsx
	20231018_NM4_cyclic_13.35kDa_mz1669_post-cIM-CID.raw.zip	Raw

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