Proteomics

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Physicochemical and proteomic characterization reveals a remodeling of Escherichia coli and Proteus mirabilis outer membrane vesicles obtained under culture conditions mimicking urine growth


ABSTRACT: Uropathogenic bacteria present a variety of mechanisms that allow them to colonize the urinary tract. These mechanisms include biofilm formation, urothelial cell invasion, and the production of adhesins, toxins, and siderophores. Uropathogenic Escherichia coli and Proteus mirabilis are two of the most common etiological agents causing urinary tract infections (UTI). In addition to virulence factors, Gram-negative bacteria can produce outer membrane vesicles (OMVs). The OMVs may have several functions in the context of UTI. In the present work, we isolated and characterized OMVs from two clinical strains: E. coli U144 and P. mirabilis 2921 cultured in Luria-Bertani broth and artificial urine. The OMVs were examined using DLS, NTA and TEM and found to be between 85 and 260 nm in size, the largest being those obtained in artificial urine. All OMVs had a low polydispersity factor and negative charge in their surface. Through proteomic analysis (nanoLC-MS/MS), 282 and 353 proteins were identified in OMVs obtained from E. coli and P. mirabilis LB cultures respectively, and 215 and 103 proteins when they were grown in AU. The majority of proteins were from the bacterial envelope. Also, several proteins related to motility and adhesion were found. The composition of OMVs proteins was different according to the culture medium. Among the identified proteins, those related with zinc and iron uptake systems could have an important role in AU. These results bring us closer to understanding the possible role of OMVs in the pathogenesis of UTIs.

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Escherichia Coli Proteus Mirabilis

TISSUE(S): Vesicle

DISEASE(S): Urinary Tract Infection

SUBMITTER: Analía Lima  

LAB HEAD: Paola Scavone

PROVIDER: PXD052681 | Pride | 2024-10-25

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
230526_Ecoli_AU_rep1.raw Raw
230526_Ecoli_AU_rep2.raw Raw
230526_Ecoli_AU_rep3.raw Raw
230526_Ecoli_LB_rep1.raw Raw
230526_Ecoli_LB_rep2.raw Raw
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