Proteomics

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Effect of lower formic acid concentration in the mobile phase in LC-MS proteomic experiments

ABSTRACT: The primary aim of this study was to evaluate the effect of lower formic acid concentration in the mobile phase on the LC separation and MS signal response in peptide separation using analytical columns packed with charged surface hybrid (CSH) stationary phase.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Francisella Tularensis Subsp. Holarctica (strain Lvs)

TISSUE(S): Cell Culture

SUBMITTER: Juraj Lenco  

LAB HEAD: Dr Juraj Lenco, Ph.D.

PROVIDER: PXD044781 | Pride | 2024-08-08

REPOSITORIES: Pride

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RK088_HeLa_100ng_Direct_inj_UserProg_1ul_0p01FA_02.raw Raw
RK088_HeLa_100ng_Direct_inj_UserProg_1ul_0p01FA_03.raw Raw
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Publications

A stationary phase with a positively charged surface allows for minimizing formic acid concentration in the mobile phase, enhancing electrospray ionization in LC-MS proteomic experiments.

Jadeja Siddharth S   Kupcik Rudolf R   Fabrik Ivo I   Sklenářová Hana H   Lenčo Juraj J  

The Analyst 20231120 23

The default choice of mobile phase acidifier for bottom-up LC-MS proteomic analyses is 0.10% formic acid because of its decent acidity, decent ion pairing ability, and low suppression of electrospray ionization. In recent years, state-of-the-art columns have been designed specifically to provide efficient separation even when using an MS-friendly mobile phase of low ionic strength. Despite this, no attempts have been made to improve the sensitivity of the MS-based analytical methods by reducing  ...[more]

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