Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The quantitative proteome of B. subtilis DK1042 strain and DS7906 strain

ABSTRACT: This project is about the proteomes of DS7906 and DK1042

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Bacillus Subtilis

TISSUE(S): Cell Culture

SUBMITTER: Manlu Zhu

LAB HEAD: Xiongfeng Dai & Manlu Zhu

PROVIDER: PXD053191 | Pride | 2024-11-02

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	XB01826LQ_A1_Slot2-35_1_33095.d.zip	Other
	XB01826LQ_A2_Slot2-36_1_33096.d.zip	Other
	XB01826LQ_B1_Slot2-37_1_33098.d.zip	Other
	XB01826LQ_B2_Slot2-38_1_33099.d.zip	Other
	XB01826LQ_C1_Slot2-39_1_33101.d.zip	Other

Items per page:

1 - 5 of 10

Similar Datasets

the quantitative proteome of B. subtilis strain 168 and its RapP-overexpressing strain

Project description:This project is about the proteomes of 168 and its RapP-overexpressing strain

2024-11-02 | PXD053195 | Pride

study on the ppGpp-mediated stringent response of E. coli during nutrient downshift

Project description:There are three projects including AE155LQ, RE161LQ, RE235LQ. AE155LQ is about the proteomes of wild type cells (WT) and E. coli RelA* OE strain during exponential growth in glucose cAA medium. E1 & E2 corresponds to the wild type cells (two biological replicate samples) and E3 & E4 corresponds to RelA* OE data (two biological replicate samples). RE161LQ is about the time-course proteome of wild type (four samples including WT_0, WT_20, WT_40 and WT_80) vs relA-deficient strain (relA_0, relA_1.5 h, relA_3 h and relA_4.5 h) during AA downshift. RE235Q is about the time-course proteome of wild type (three samples including WT_0, WT_60, WT_160) vs relA-deficient strain (three samples including relA_0, relA_60 and relA_160) during carbon downshift.

2023-01-26 | PXD035675 | Pride

E.coli (p) ppGpp regulation.E. coli, ppGpp, growth rate

Project description:These two projects are about the proteome of wild type E. coli K-12 NCM3722 strain (RE198LQ) and its rpoS-null strain (XA00994LQ). Both projects include replicates of two conditions, the normal conditions and (p)ppGpp overproduction conditions via RelA* OE.

2023-12-20 | PXD042250 | Pride

Bacillus subtilis spo0A trade-off

Project description:This project is about the proteomes of wild type cells (WT) and spo0A-null strain of B. subtilis during exponential growth in mannose medium. B2 & B4 corresponds to the wild type cells (two biological replicate samples) and B6 & B8 corresponds to spo0A-null data (two biological replicate samples).

2023-05-10 | PXD039192 | Pride

Melatonin promotes chilling tolerance of cucumber seedlings by regulating antioxidant systems and relieving photoinhibition

Project description:Chilling is a major stress which adversely decreases photosynthesis and leads to oxidative stress in thermophilic plants. The role of melatonin (MT) in stress response of plants, has so far been investigated, while the mechanisms by which MT regulate photosynthesis in chilling-sensitive cucumber under chilling stress remain unclear. In this paper, we reported MT positively regulated chilling tolerance of cucumber. Moreover, MT showed a concentration-dependent manner and 1.0 μmol·L-1 was the optimum concentration, of which the chilling injury index, EL and MDA were the lowest, while growth was the highest among all the treatments. Further studies indicated that MT triggered the activity and expression of the antioxidant enzymes, which in turn decreased ROS accumulation caused by chilling stress. Meanwhile, MT maintained a high photosynthetic carbon assimilation capacity, increased the activity of PSI reaction center, PSII reaction center and electron transfer efficiency. More importantly, the proteome analysis and western-blot data revealed that PSI-associated proteins (PsaD, PsaE, PsaF, PsaH and PsaN), PSII-associated protein D1 as well as Rubisco large subunit and RCA were significantly up-regulated in MT-treated seedlings, compared to H2O-treated seedlings under chilling stress. These results suggest that MT enhances chilling tolerance of cucumber via activating the antioxidant system as well as protein level of key PSI-, PSII-related and carbon assimilation enzymes to decrease ROS accumulation, and finally alleviates the damage to photosynthetic apparatus under chilling stress.

2022-02-15 | PXD029134 | Pride

MeDIP-chip from head and neck squamous cell carcinoma tissues and normal subjects

Project description:Profiling the genome-wide methylation status of head and neck squamous cell carcinoma and normal subjects Screening HNSCC-related candidate methylation gene by comparison of HNSCC tissues vs normal subjects.

2014-06-19 | E-GEOD-58630 | biostudies-arrayexpress

Tuberculosis alters immune-metabolic pathways resulting in perturbed IL-1 responses [metabolomics]

Project description:Tuberculosis (TB) remains a major public health problem and we lack a comprehensive understanding of how Mycobacterium tuberculosis (M. tb) infection impacts host immune responses. We compared, at two timepoints, the induced immune response to TB antigen, BCG and IL-1β stimulation between latently M. tb infected individuals (LTBI) and active TB patients. The immune response was assessed using the TruCulture system with a Null stimulation. samples were tested by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectroscopy (UPLC MS/MS) for a total of 696 metabolites.

2022-11-05 | E-MTAB-12381 | biostudies-arrayexpress

ECBiG PlasTi-microarray CSH validation

Project description:Experiment evaluating aplicability of PlasTi-microarray for cross-species hybridization studies. PlasTi-microarray is a tiling oligonucleotide microarray originally designed for cucumber plastome analysis.Chloroplast RNA from Arabidopsis, tomato and spinach leaves was extracted, labelled and hybridized to PlasTi-microarray with the cucumber samples labelled with the second dye as a control. For each species, one biological sample and one technical replicate (labelled in a dye-swap orientation) were analyzed, resulting in two microarray hybridizations per species and six microarrays (a-f) in total.

2011-05-27 | E-MEXP-3220 | biostudies-arrayexpress

Identification of Proteins in Streptococcus pneumoniae extracellular vesicles (pEVs)

Project description:Extracellular vesicles (EVs) have recently garnered attention for their participation in host-microbe interactions in Streptococcus pneumoniae infections. However, the effect of pEVs on the disruption of alveolar epithelial barrier remain poorly understood. Our studies focus on EVs produced by Streptococcus pneumoniae (pEVs), and reveal that pEVs are internalized by alveolar epithelial cells. In vitro, pEVs induce autophagy activation in a dosage-dependent manner and decrease the alveolar epithelial barrier’s trans-epithelium electrical resistance (TEER). In addition, pEV-containing bacterial peotein serine/threonine-protein kinase StkP may act as an activator for Streptococcus pneumoniae-induced autophagy activation. When administered systemically in mice, Streptococcus pneumoniae wild type strain induced acute lung injury, the deletion of stkP deletion strain attenuated this injury. Taken together, pEVs cargos emerge as critical contributors to tissue damage in mammalian hosts.

2023-12-13 | PXD046576 | Pride

quantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7

Project description:Clostridium sp. strain CT7 is a new emerging microbial cell factory with high butanol ratio owing to the non-traditional butanol fermentation mode with uncoupled acetone and 1,3-propanediol formation. Significant change of products profile was shown in glycerol- and glucose-fed strain CT7, especially much higher butanol and lower volatile fatty acids production from glycerol-fed one. However, the mechanism of this interesting phenomenon was still unclear. To better elaborate the bacterial response towards glycerol and glucose, the quantitative proteomic analysis through iTRAQ strategy was performed to reveal the regulated proteomic expression levels under different substrates. Proteomics data showed highly increased proteomic expression levels of proteins related with glycerol utilization and solvent generation under glycerol media. In addition, the up-regulation of hydrogenases, ferredoxins and electron-transferring proteins may attribute to the internal redox balance, while the earlier triggered sporulation response in glycerol-fed media may be associated with the higher butanol fermentation. This study will provide the platform for metabolic engineering of this emerging industrial microorganism for more efficient butanol production from glycerol.

2021-09-08 | PXD016848 | Pride