Proteomics

Dataset Information

0

Proteomic analysis of sap reveals root-shoot communication dynamics in potato and barley following flg22 treatment


ABSTRACT: Potato (Solanum tuberosum, variety Princ) and barley (Hordeum vulgare, variety Sebastian) plants were cultivated in a controlled environment. Plants were grown in Potgrond H soil (Klasmann-Deilmann GmbH, Germany) under a 12-hour photoperiod with a constant temperature of 21 °C and a photon flux density of 100 μmol m⁻² s⁻¹. Potato tubers were used as starting material, while barley plants were grown from surface-sterilized seeds. After six weeks of cultivation for potato and three weeks for barley, the sap proteome was collected by cutting the stems 10–20 mm above the soil surface. Root exudates were sampled into three fractions over a two-hour period: F1 (15 min), F2 (75 min), and F3 (135 min). Each experiment included at least three independent biological replicates. A subset of plants was pre-incubated with flg22, a conserved peptide sequence derived from bacterial flagellin (QRLSTGSRINSAKDDAAGLQIA, >95% purity; ProteoGenix, France). Flg22 solution (1 μM flg22, 0.025% v/v Silwet L-77) was applied by spraying the leaves and pouring the solution under the pot. Potato plants received 30 ml and 200 ml of the solution for spraying and watering, respectively. Due to their smaller size, barley plants were treated with half the solution volume. Mock-treated plants received a solution containing only 0.025% Silwet L-77. After 24 hours, plants were cut, and the third fraction of the sap (75-135 min) was collected. Root and shoot tissues were collected in liquid nitrogen, the sap was collected as described above. Each experiment included at least three independent biological replicates.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Hordeum Vulgare (barley) Solanum Tuberosum (potato)

TISSUE(S): Xylem, Root, Shoot

SUBMITTER: Martin Cerny  

LAB HEAD: Martin Cerny

PROVIDER: PXD053406 | Pride | 2024-07-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
RK-Solanum-FLG22-01-F03.msf Msf
RK-Solanum-FLG22-01-F03.raw Raw
RK-Solanum-FLG22-02-F03.msf Msf
RK-Solanum-FLG22-02-F03.raw Raw
RK-Solanum-FLG22-03-F03.msf Msf
Items per page:
1 - 5 of 86

Similar Datasets

2011-10-05 | E-GEOD-32642 | biostudies-arrayexpress
2007-09-01 | GSE8161 | GEO
2021-05-03 | PXD022913 | Pride
2016-09-01 | GSE84277 | GEO
2010-12-11 | E-GEOD-14053 | biostudies-arrayexpress
2010-03-02 | E-GEOD-17464 | biostudies-arrayexpress
2014-10-23 | E-GEOD-56333 | biostudies-arrayexpress
2011-11-22 | GSE33405 | GEO
2012-01-01 | GSE23184 | GEO
2007-09-01 | GSE8208 | GEO