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Nanosenors containing nonstandard amino acids

ABSTRACT: High resolution mass spectrometry is used to confirm conjugation of fluorogenic nonstandard amino acids onto an intermediate molecule, and further verify site-specific ribosomal incorporation into different positions of a polypeptide.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Escherichia Coli

SUBMITTER: Allison Flores

LAB HEAD: George Church

PROVIDER: PXD053593 | Pride | 2024-10-17

REPOSITORIES: Pride

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			Action	DRS
	72016_89907.mzid.gz	Mzid
	72016_89907.pep.xml	Pepxml
	72022_89908.mzid.gz	Mzid
	72022_89908.pep.xml	Pepxml
	72023_89910.mzid.gz	Mzid

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Publications

Rapid discovery and evolution of nanosensors containing fluorogenic amino acids.

Kuru Erkin E Rittichier Jonathan J de Puig Helena H Flores Allison A Rout Subhrajit S Han Isaac I Reese Abigail E AE Bartlett Thomas M TM De Moliner Fabio F Bernier Sylvie G SG Galpin Jason D JD Marchand Jorge J Bedell William W Robinson-McCarthy Lindsey L Ahern Christopher A CA Bernhardt Thomas G TG Rudner David Z DZ Collins James J JJ Vendrell Marc M Church George M GM

Nature communications 20240905 1

Binding-activated optical sensors are powerful tools for imaging, diagnostics, and biomolecular sensing. However, biosensor discovery is slow and requires tedious steps in rational design, screening, and characterization. Here we report on a platform that streamlines biosensor discovery and unlocks directed nanosensor evolution through genetically encodable fluorogenic amino acids (FgAAs). Building on the classical knowledge-based semisynthetic approach, we engineer ~15 kDa nanosensors that reco ...[more]

PMID: 39237489

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