Dataset Information

Proteome of claws and skin of Anolis carolinensis and Pogona vitticeps

ABSTRACT: Proteomic analysis of Anolis and Pogona claws. The claw proteome will be compared to regular skin.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Anolis Carolinensis Pogona Vitticeps

TISSUE(S): Skin, Claw

SUBMITTER: Attila Placido Sachslehner

LAB HEAD: Prof.Dr. Leopold Eckhart

PROVIDER: PXD054063 | Pride | 2024-08-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	240717_IP_ASa_040-V0051-A02-X0310-Ac_claw_Peptides.xlsx	Xlsx
	240717_IP_ASa_040-V0051-A02-X0310-Ac_claw_Proteins.xlsx	Xlsx
	240717_IP_ASa_040-V0051-A03-X0311-Ac_BSK_Peptides.xlsx	Xlsx
	240717_IP_ASa_040-V0051-A03-X0311-Ac_BSK_Proteins.xlsx	Xlsx
	240717_IP_ASa_040-V0051-A04-X0312-Ac_BeSK_Peptides.xlsx	Xlsx

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Project description:The autoinflammatory disorder, Neonatal-onset Multisystem Inflammatory Disease (NOMID) is the most severe phenotype of disorders caused by mutations in the CIAS1 gene that result in increased production and secretion of active IL-1≤. NOMID patients present with systemic and organ-specific inflammation of the skin, central nervous system and bony growth plates, and respond dramatically to treatment with IL-1 blocking agents. We compared the cellular infiltrates and transcriptome of skin biopsies from patients with NOMID (n=14) before treatment [lesional (LS) and non-lesional (pre-NL) skin] and after treatment (post-NL) with the IL-1 blocker anakinra (recombinant IL-1 receptor antagonist, Kineret®), to normal skin (n=5). Abundant neutrophils distinguish LS skin from pre-NL and post-NL skin. CD11c+ dermal dendritic cells and CD163+ macrophages expressed activated caspase-1 and are the likely sources of cutaneous IL-1 production. Treatment with anakinra led to the disappearance of neutrophils, but CD3+ T cells and HLA-DR+ cells remained elevated. Among the upregulated genes IL-6, IL-8, TNF, IL-17A, CCL20, and the neutrophil defensins DEFA1 and DEFA3 were differentially regulated in LS tissues (compared to normal skin). Important significantly downregulated pathways included IL-1R/TLR signaling, type I and II cytokine receptor signaling, mitochondrial dysfunction, and antigen presentation. Differential expression and regulation of microRNAs and pathways involved in post-transcriptional modification are indicative of epigenetic modification in the various tissue states. Overall, the dysregulated genes and pathways suggest extensive “adaptive” mechanisms to control inflammation, and maintain tissue homeostasis in the skin of patients with NOMID. To determine the transcsriptome of skin samples in Neonatal-onset Multisystem Inflammatory Disease (NOMID), using pre-treatment non-lesional (pre-NL, n=4); lesional (LS, n=6); post-treatment non-lesional (post-NL, n=8), and normal skin (n=5). Comparison of mean gene expression of each group at baseline, and considering treatment effect

Dataset Information

Proteome of claws and skin of Anolis carolinensis and Pogona vitticeps

Dataset's files

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