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Robust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - HEK293T - Global expression proteomics

ABSTRACT: In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. This platform profiles cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiobiotin (IA-DTB) in cell lysates and live cells. We employ label free quantification and data independent acquisition (DIA) on an Evosep One – Bruker timsTOF Pro. In this submission we report this use of global proteomics to explore protein expression in HEK293T.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: George Biggs

LAB HEAD: Jacob Bush

PROVIDER: PXD054105 | Pride | 2024-11-18

REPOSITORIES: Pride

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			Action	DRS
	20240326_150137_ELB00133_hek_global_v18.sne	Other
	20240429_140737_ELB00133_hek_global_v18_Report.setup.txt	Txt
	20K_HUMAN_1GENE_202210.fasta	Fasta
	ELB00131_DC2882_P03_A04_16362.d.rar	Other
	ELB00131_DC2882_P03_B04_16363.d.rar	Other

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