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Identification of proteins from Bas22 and Bas60 bacteriophages recognized by the KpAvs2 bacterial defense system during infection of Escherichia coli.

ABSTRACT: Antiviral STANDs (Avs) are bacterial anti-phage proteins that are considered as the evolutionary ancestors of immune pattern-recognition receptors of the NLR family. Following the recognition of a conserved phage protein, Avs proteins exhibit cellular toxicity and abort phage propagation by killing the infected cell. Type 2 Avs proteins (Avs2) were suggested to recognize the large terminase subunit of the phage by direct binding as a signature of phage infection based on co-expression assays. Here, we analyzed the binding partners of a type 2 Avs protein from Klebsiella pneumoniae (KpAvs2) expressed in Escherichia coli during Bas22 and Bas60 phage infection and showed that KpAvs2 recognizes both phages through binding of different proteins.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Suspension Culture

SUBMITTER: Nathalie Bechon

LAB HEAD: Rotem Sorek

PROVIDER: PXD054782 | Pride | 2024-12-04

REPOSITORIES: Pride

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			Action	DRS
	AllQuantifiedPeptides.tsv	Tabular
	AllQuantifiedProteinGroups.tsv	Tabular
	HFX_RS_21381_22-SHA-A_23052024.raw	Raw
	HFX_RS_21381_22-SHA-B_23052024.raw	Raw
	HFX_RS_21381_22-SHA-C_23052024.raw	Raw

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