Proteomics

Dataset Information

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Metabolic Adaptation in Epilepsy: From Acute Response to Chronic Impairment


ABSTRACT: Epilepsy is characterized by hypersynchronous neuronal discharges, which are associated with an increased cerebral metabolic rate of oxygen and ATP demand. Uncontrolled seizure activity (status epilepticus) results in mitochondrial exhaustion and ATP depletion, which potentially generate energy mismatch and neuronal loss. Many cells can adapt to increased energy demand by increasing metabolic capacities. However, acute metabolic adaptation during epileptic activity and its relationship to chronic epilepsy remains poorly understood. We elicited seizure-like events (SLEs) in an in vitro model of status epilepticus for eight hours. Electrophysiological recording and tissue oxygen partial pressure recordings were performed. After eight hours of ongoing SLEs, we used proteomics-based kinetic modeling to evaluate changes in metabolic capacities. We compared our findings regarding acute metabolic adaptation to published proteomic and transcriptomic data from chronic epilepsy patients. Epileptic tissue acutely responded to uninterrupted SLEs by upregulating ATP production capacity. This was achieved by a coordinated increase in the abundance of proteins from the respiratory chain and oxidative phosphorylation system. In contrast, chronic epileptic tissue shows a 25-40% decrease in ATP production capacity. In summary, our study reveals that epilepsy leads to dynamic metabolic changes. Acute epileptic activity boosts ATP production, while chronic epilepsy reduces it significantly.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Rattus Rattus (black Rat)

TISSUE(S): Brain

DISEASE(S): Seizure

SUBMITTER: Stefan Loroch  

LAB HEAD: Stefan Loroch

PROVIDER: PXD055090 | Pride | 2024-09-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20221128_085614_STrap_10STrapFractions_Rattus_sp_tr_duplicatesRemoved.sne Other
EXII00736_01.raw Raw
EXII00737_06.raw Raw
EXII00738_07.raw Raw
EXII00739_02.raw Raw
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