Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Surviving Martian Brines: Proteomic Analysis of Escherichia coli’s Adaptation to Perchlorate Stress

ABSTRACT: Brines, potentially formed by the deliquescence and freezing point depression of highly hygroscopic salts, such as perchlorates (ClO4-), may allow for the spatial and temporal stability of liquid water on present-day Mars. It is therefore of great interest to explore the microbial habitability of Martian brines, for which our current understanding is, however, still limited. Putative microbes growing in the perchlorate-rich Martian regolith may be harmed due to the induction of various stressors including osmotic, chaotropic, and oxidative stress. We adapted the model organism Escherichia coli to increasing sodium perchlorate concentrations and used a proteomic approach to identify the adaptive phenotype. Separately, the microbe was adapted to elevated concentrations of NaCl and glycerol, which enabled us to distinguish perchlorate-specific adaptation mechanisms from those in response to osmotic, ion and water activity stress. We found that the perchlorate-specific stress-response focused on pathways alleviating damage to nucleic acids, presumably caused by increased chaotropic and/ or oxidative stress. The significant enrichments that have been found include DNA repair, RNA methylation and de novo IMP biosynthesis. Our study provides insights into the adaptive mechanisms necessary for microorganisms to survive under perchlorate stress, with implications for understanding the habitability of Martian brines.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Escherichia Coli

SUBMITTER: Joerg Doellinger

LAB HEAD: Peter Lasch

PROVIDER: PXD055273 | Pride | 2025-01-21

REPOSITORIES: pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DiaNN.zip	Other
	Ecoli_stress-response-lib.predicted.speclib	Other
	Gly_1.raw	Raw
	Gly_2.raw	Raw
	Gly_3.raw	Raw

Items per page:

1 - 5 of 15

Similar Datasets

Perchlorate-Specific Proteomic Stress Responses of Debaryomyces hansenii Could Enable Microbial Survival in Martian Brines

Project description:If life exists on Mars, it would face several challenges including the presence of perchlorates, which destabilize biomacromolecules by inducing chaotropic stress. However, little is known about perchlorate toxicity for microorganism on the cellular level. Here we present a proteomic investigation on the perchlorate-specific stress responses of the halotolerant yeast Debaryomyces hansenii and compare these to generally known salt stress adaptations. We found that the responses to NaCl and NaClO4-induced stresses share many common metabolic features, e.g., signaling pathways, elevated energy metabolism, or osmolyte biosynthesis. However, several new perchlorate-specific stress responses could be identified, such as protein glycosylation and cell wall remodulations, presumably in order to stabilize protein structures and the cell envelope. These stress responses would also be relevant for life on Mars, which - given the environmental conditions - likely developed chaotropic defense strategies such as stabilized confirmations of biomacromolecules and the formation of cell clusters.

2023-06-06 | PXD033237 | Pride

Gene expression during hyper and hypoosmotic stress adaptation in Gillichthys mirabilis

Project description:This study investigates changes in gene expression associated with adaptation to acute hyper- and hypoosmotic stress in Gillichthys mirabilis gill tissue. Gene expression data was captured as a time course (1, 2, 4, 12 hours past exposure) during the first 12 hours following abrupt transfer to osmotic stress media. We employed a custom made Gillichthys mirabilis cDNA microarray. A total of 39 arrays were used in this study: 12 hyperosmotic stress samples, 12 hypoosmotic stress samples, 12 control samples, and 3 time=0 samples. Three individuals were hybridized at each of the four time points (1, 2, 4, 12 hours past exposure) for experimental and control fish (giving a total of 12). All samples were hybridized against a common reference sample containing pooled cDNAs from several individuals.

2008-06-24 | E-GEOD-11700 | biostudies-arrayexpress

Expression data from porcelain crabs following heat or cold stress

Project description:Porcelain crabs, Petrolisthes cinctipes, live in the marine intertidal zone and routinely experience thermal stress. Genes involved in heat shock responses are generally upregulated following heat stress, differentially expressed genes are involved in different cellular functions. We used microarrays to detail the global programme of gene expression underlying responses to thermal stress and identified distinct classes of up-regulated and down-regulated genes during this process. Crabs were collected from the field and returned to the laboratory where they were given a heat or cold stress or held under control conditions for the period of time during the thermal stress.

2009-09-21 | E-GEOD-17361 | biostudies-arrayexpress

Early drought-responsive changes in pepper leaves and roots

Project description:Drought is one of the major factor that limits crop production and reduces yield. To understand the early response of plants under nearly natural conditions, pepper plants were grown in a greenhouse and drought stressed by withholding water for one week. Plants adapted to the decreasing water content of the substrate by adjustment of their osmotic potential in roots by accumulation of raffinose, glucose, galactinol and proline. In contrast in leaves levels of fructose, sucrose and also galactinol increased. Due to the water deficit cadaverine, putrescine, spermidine and spermine accumulated in leaves whereas the concentration of polyamines was reduced in roots. These polyamines are suggested to rather act as stress protectants than for osmotic adjustment. To understand the molecular basis of the response to this early drought stress better, four suppression subtractive hybridisation libraries from leaves and roots were constructed. Microarray technique was used to identify differentially expressed genes. A total of 109 unique ESTs were detected. The diversity of the putative functions of all identified genes confirms the complexity of the plant response to drought stress. Keywords: Transcription profiling Two-condition experiment in roots and leaves, control leaves (CL) vs. drought-stressed leaves (DL) and control roots (CR) vs. drought-stressed roots (DR). Biological replicates: 4 control (1-4), drought-stressed (1-4), independently grown and harvested. One swap replicate per array.

2010-06-20 | E-GEOD-18627 | biostudies-arrayexpress

Dynamic transcriptome analysis reveals mRNA synthesis and decay in yeast

Project description:To obtain rates of mRNA synthesis and decay in yeast, we established dynamic transcriptome analysis (DTA). DTA combines non-perturbing metabolic RNA labeling with dynamic kinetic modeling. DTA was used to monitor the cellular response to osmotic stress in comparison to the wild type. Genomic occupancy profiling of RNA polymerase (Pol) II was used to predict changes in mRNA synthesis rates.

2011-11-01 | E-MTAB-439 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:AtGenExpress: Stress Treatments (Osmotic stress)

| PRJNA96923 | ENA

Unveiling Adaptive Molecular Mechanisms Through Comparative Proteomics of Potato Cell Responses to Abrupt and Gradual Water Deficits

Project description:Osmotic stress, such as drought and salinity, impairs plant growth, productivity, and food quality. Plants cope with these stresses by modulating biological processes and molecular mechanisms based on the stress intensity and duration. Understanding how plants respond and adapt at the molecular level is crucial for developing tolerant genotypes capable of surviving and produce in harsh conditions. The cultivated potato Solanum tuberosum is the most important non-grain crop worldwide and is often considered water deficit sensitive, thus necessitating efforts to enhance their resilience. Previously, we established a reliable in vitro model using potato suspension cells to simulate sudden (shock) or gradual adaptation to water deficit. Our previous researches revealed distinct cellular and molecular responses between shocked and adapted cells. In this study, we utilized mass spectrometry-based proteomics to examine protein expression in order to capture novel functional information and identify regulatory networks during gradual adaptation compared to abrupt water stress. Remarkably, both adapted and shocked conditions triggered significant alterations in protein expression related to various metabolic pathways, including carbon metabolism, osmotic regulation, and antioxidant defense. However, adapted cells exhibited increased levels of proteins associated with fatty acid biosynthesis and metabolism, underscoring the importance of fatty acids in regulating potato cell adaptation to water deficit. Furthermore, we identified proteins involved in transcriptional and translational machinery, as well as chromatin organization, which were specifically expressed in adapted cells. This suggests a pivotal role for epigenetic changes, warranting further investigation. Importantly, our findings present a valuable list of candidate proteins for future functional analysis to elucidate their roles in plant stress response and adaptive mechanisms.

2024-12-06 | PXD050665 | Pride

Salmonella under environmental stress

Project description:The aim of this study is to investigate the transcriptional response of S. Typhimurium to heat, osmotic, oxidative and acid stress under anoxic and oxic conditions and to non-stressed anoxic conditions.

2013-11-11 | E-MTAB-1804 | biostudies-arrayexpress

Osmotic stress on yeast S. cerevisiae

Project description:mRNA amount (RA) and Transcription rate (TR) analysis of W303-1a (wt) and hog1 mutant yeast strains growing in exponential phase in YPD subjected to osmotic stress This SuperSeries is composed of the following subset Series: GSE13096: Transcription rate analysis of wild type strain subjected to osmotic stress GSE13097: mRNA amount analysis of wild type strain subjected to osmotic stress GSE13098: Transcription rate analysis of W303 hog1 mutant strain subjected to osmotic stress GSE13099: mRNA amount analysis of W303 hog1 mutant strain subjected to osmotic stress Refer to individual Series Transcriptomic and transcription rate analysis by means of GRO of three independent replicates the yeast strain growing in exponential phase. Each time point replicate has been hybridized on a different macroarray (F11-F24). A single DNA genomic hybridization from the same labeling reaction was done on the same microarrays for normalization.

2010-06-19 | E-GEOD-13100 | biostudies-arrayexpress

Differentiation of human embryonic stem cells towards hepatocytes

Project description:To understand in detail the molecular phases that occur during the differentiation of human embryonic stem cells to hepatocyte-like cells we performed an RNA-seq time course of the stages of differentiation across the 21 day differentiation time course. We used a chemically defined (serum-free) protocol, modified from Si-Tayeb et al., 2010 and Song et al., 2009. We describe acquisition of definitive endoderm characteristics at day 3, followed by hepatoblast charcter at day 7-13 and then maturation to hepatocyte-like cells at day 21. Time course of differentiation of human embryoic stem cells to hepatocyte-like cells over 21 days, using an adapted chemically defined protocol of Song et al., 2009; Cell Research

2017-04-28 | E-GEOD-70741 | biostudies-arrayexpress