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C-terminal amides mark proteins for degradation via SCF/FBXO31 – CTAP discovery

ABSTRACT: FBXO31 is a substrate adapter for the SCF-type ubiquitin ligase complex SCF/FBXO31 which ubiquitylates C-terminal amide-bearing proteins (CTAPs), thereby triggering their proteasomal degradation in human cells. CTAPs can arise from internal protein cleavage events during which the N-terminal fragment is left with an amidated C-terminus (-0.984016 Da). We searched for such cleavage events (semi-enzymatic C-terminus and C-terminal amidation) in previously published datasets and in own experimental data. These include: - Re-analysis of tissue samples from a deep human proteome atlas (PXD010154) - IP-MS of AARS1, a client of the CTAP reader FBXO31 (MFM-X208) - In vitro fragmentation of purified hemoglobin (MFM-X294, MFM-X298) and tRNA ligase complex (MFM-X314) Experiments are labeled with experiment IDs (MFM-X...). PSM tables contain Experiment IDs and interpretable sample aliases.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart, Brain, Cell Culture, Adipose Tissue, Thyroid Gland

SUBMITTER: Matthias Muhar

LAB HEAD: Jacob Corn

PROVIDER: PXD055814 | Pride | 2024-11-05

REPOSITORIES: Pride

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			Action	DRS
	20221124_C30067_006_S426614_MFM-X208-2_Group_2.raw	Raw
	20240313_C34501_002_S648537_MFM-X294-1_Group_1.raw	Raw
	20240313_C34501_004_S648538_MFM-X294-2_Group_2.raw	Raw
	20240313_C34501_006_S648539_MFM-X294-3_Group_3.raw	Raw
	20240313_C34501_008_S648540_MFM-X294-4_Group_4.raw	Raw

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