Transcriptomics

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Functional analysis of caspase cleavable proteoforms of the Drosophila GSK-3 gene shaggy.


ABSTRACT: A comparison of the embryonic transcriptome of wild type embryos (sggisoD-DS-SFS ) with embryos harbouring mutated DEVD caspase recognition sites ( sggisoD-cI-DS-SFS ) in a major shaggy isoforms class to examine the role of caspase cleavage in sgg function. The unique N-terminal exon undegoing caspase cleavage is tagged with DsRED at its N-terminus and StrepII-Flag-StrepII upstream of caspase cleavage sites for the wild type and caspase insensitive variant versions.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE157625 | GEO | 2020/09/09

REPOSITORIES: GEO

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