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Mechano-transduction by LIM domain famliy protein LPP Drives Tumor Progression

ABSTRACT: To identify the protein interactome of LPP, we performed BioID mass spectrometry to detect the interacting proteins. HEK293T cells were transfected with BirA*-LPP for 24 hours, then cells treated with 50 µM biotin or without biotin were incubated with 50 µl of streptavidin-agarose beads and mass spectrometry was performed.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Man Yu

LAB HEAD: Shuguo Sun

PROVIDER: PXD056010 | Pride | 2025-04-02

REPOSITORIES: Pride

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			Action	DRS
	22042003_YM_LPP-Biotin.raw	Raw
	22042003_YM_LPP-NC.raw	Raw
	Biotin.peptides.xlsx	Xlsx
	Biotin.proteins.xlsx	Xlsx
	NC.peptides.xlsx	Xlsx

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A mechanism of YAP/TAZ transactivation and dual targeting for cancer therapy

Project description:Transcriptional coactivators of YAP/TAZ play key roles in cancers through transcriptional outputs. However, the mechanisms of their transactivation remain unclear, and effective and safe targeting solutions are lacking. Here, we discovered that YAP/ TAZ possess a hydrophobic transactivation domain (TAD). Knockout of TADs prevents tumor establishment due to growth defects and enhanced immune attack. TADs facilitate preinitiation complex (PIC) assembly by promoting TFIID recruitment in a TAF4-dependent manner and enhance RNA polymerase II (Pol II) elongation through MED15-dependent mediator complex recruitment. The bindings of TAD to the surface hydrophobic groove of MED15 induce its folding of the helix and hydrophobic interactions of TAD-MED15 boosts YAP-MED15 co-condensations thereby enhancing transcriptional hub formation and the expression of an oncogenic and immune suppressive transcriptional program. Synthesized small peptide TJ-M11 selectively disrupts the interactions of TADs with MED15 and TAF4, thereby suppressing tumor growth and sensitizing immunotherapy. This study indicates TADs of YAP/TAZ exhibit dual functions in PIC assembly and Pol II elongation, relying on hydrophobic interactions.

2025-04-02 | PXD060955 | Pride

The ShcA adapter protein cooperates with LPP to mediate adhesion dynamics and invadopodia formation

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Identification of proteins that interact YAP TAD or MED15 through BioID

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Lichen Planopilaris and Pseudopelade of Brocq Involve Distinct Disease Associated Gene Expression Patterns by Microarray

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