Dataset Information

Impact of mutations in the HEADING DATE 1 gene on transcription and cell wall composition of rice_P2777

ABSTRACT: HEADING DATE 1 (Hd1) is a zinc finger transcription factor, central in the photoperiodic flowering network. Hd1 operates as a repressor of flowering under long days but reverts to a promoter of flowering under short days. How this dual function is organized at the proteomic level is still unknown. Here, we have used MS to interrogate the proteome of hd1 mutants and wild type plants under long and short day conditions, and we identified differential abundance of proteins involved in cell wall metabolism.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Frank Stein

LAB HEAD: Camilla Betti

PROVIDER: PXD056444 | Pride | 2025-04-10

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	FragPipe_workflow.workflow	Other
	P2777_fractionated_ion.tsv	Tabular
	P2777_fractionated_peptide.tsv	Tabular
	P2777_fractionated_protein.tsv	Tabular
	P2777_fractionated_psm.tsv	Tabular

Items per page:

1 - 5 of 22

Publications

Mutations in HEADING DATE 1 affect transcription and cell wall composition in rice.

Biancucci Marco M Chirivì Daniele D Baldini Alessio A Badenhorst Eugene E Dobetti Fabio F Khahani Bahman B Formentin Elide E Eguen Tenai T Turck Franziska F Moore John P JP Tavakol Elahe E Wenkel Stephan S Lo Schiavo Fiorella F Ezquer Ignacio I Brambilla Vittoria V Horner David D Chiara Matteo M Perrella Giorgio G Betti Camilla C Fornara Fabio F

Plant physiology 20250327

Plants utilize environmental information to modify their developmental trajectories for optimal survival and reproduction. Over a century ago, day length (photoperiod) was identified as a major factor influencing developmental transitions, particularly the shift from vegetative to reproductive growth. In rice (Oryza sativa), exposure to day lengths shorter than a critical threshold accelerates flowering, while longer days inhibit this process. This response is mediated by HEADING DATE 1 (Hd1), a ...[more]

PMID: 40152517

Similar Datasets

Project description:Intracellular bacterial pathogens inject a cocktail of effector proteins into host cells to hijack diverse cellular processes and promote their survival and proliferation. Salmonella enterica serovar Typhimurium (STm) uses two Type 3 secretion system (T3SS) to deliver a suite of effector proteins into host cells, however, knowledge of their host-targets remains sparse. To systematically map STm effector-host protein-protein interactions (PPIs) during active infection, we generated a library of 32 STm strains expressing affinity-tagged effector proteins from their endogenous chromosomal loci. Tagged-effector strains were then used to infect macrophages or epithelial cells, and PPIs were detected by Affinity-Purification Quantitative Mass-Spectrometry (AP-QMS) under both native and cross-linking conditions. We recovered 25 previously described effector-host PPIs, along with 421 novel interactions across the two cell lines tested, as well as several effector-effector PPIs. While effectors converged on specific host cellular processes, which varied across cell types, most had multiple host targets. Using reciprocal co-immunoprecipitations, we could validated 13 out of 22 new PPIs, demonstrating an accuracy of at least 59% for the AP-QMS approach. To illustrate the utility of this resource for discovering novel infection biology, we first showed that SseJ and SseL cooperate to control cholesterol transport at the Salmonella Containing Vacuole (SCV) via the Niemann-Pick C1 protein (NPC1). Second, we uncovered that PipB directly binds and recruits the organelle contact site protein PDZD8 to the SCV. Third, we elucidated a novel mechanism whereby the effector kinase SteC promotes host actin bundling, by directly interacting and phosphorylating formin-like proteins. Overall, we provide a systems-wide host-bacterial pathogen physical interactome resource, to our knowledge the first in an infection context, with broad implications for effector function and cooperation.