Dataset Information

Immunoprecipitation of U2AF2-TurboID-HA

ABSTRACT: Analysis of U2AF2-TurboID-HA interactions by immunoprecipitation for comparison with proximity labeling. Cells expressing U2AF2 (iU2) fused with TurboID in either the undifferentiated state (D0) or after mesoderm differentiation (D1) were analyzed to identify the interactome and detect changes during the differentiation process.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell

SUBMITTER: Rubens Gomes Junior

LAB HEAD: Bruno Dallagiovanna

PROVIDER: PXD062890 | Pride | 2025-04-13

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20250402_43125_Rubens_HAU2D0R1_1.raw	Raw
	20250402_43125_Rubens_HAU2D0R1_2.raw	Raw
	20250402_43325_Rubens_HAU2D0R3_1.raw	Raw
	20250402_43325_Rubens_HAU2D0R3_2.raw	Raw
	20250402_43425_Rubens_HAU2D0R4_1.raw	Raw

Items per page:

1 - 5 of 27

Similar Datasets

Project description:Cardiovascular diseases are a major cause of life-threatening burden around the world. The heart has a very low regeneration capacity and donor organs for transplantation are scarce. Therefore regeneration of lost myocardium with stem cell-derived cardiomyocytes (CMs) provides an attractive strategy for heart repair. Human pluripotent stem cells (hPSCs) can be efficiently differentiated in vitro into CMs but the molecular mechanisms behind this process are still not fully understood. In particular identification of secreted autocrine and/or paracrine factors that function as important extrinsic signals remained elusive because the mass spectrometry (MS)-based identification of secreted proteins from cell culture supernatants is impeded by high levels of albumin present in common differentiation media. Thus we established an albumin-free cardiomyogenic differentiation medium and performed secretomics at seven different time points during in vitro differentiation. This analysis led to the identification of 4832 proteins with 1802 being significantly altered during differentiation and 431 of these were annotated as secreted according to gene ontology. Bioinformatics revealed enrichment of extrinsic Wnt pathway-related proteins 3 days upon induction of differentiation and of extracellular matrix proteins in the resulting CMs. Numerous extrinsic components of Wnt, Activin A, Nodal, TGFβ, BMP or FGF signaling pathways were quantitatively assessed during differentiation. Notably, the abundance of pathway agonists was generally lower compared to the respective antagonists but their curves of progression over timer were rather similar. We hypothesize that Activin A, Nodal and TGFβ signaling are turned down shortly upon initiation of cardiac differentiation whereas BMP signaling is switched on. Wnt and FGF signaling peaks between d0 and d3 of differentiation and interestingly, Activin A and TGFβ signaling seem to be reactivated at the cardiac progenitor stages and/or in early CMs.

Dataset Information

Immunoprecipitation of U2AF2-TurboID-HA

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets