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Lacticaseibacillus rhamnosus GG strain:ATCC 53103

ABSTRACT: Lactobacillus rhamnosus GG strain ATCC 53103 genome sequencing project

PROVIDER: PRJDA40637 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	GCA_000011045.1_sequence_report.txt	Txt

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Lacticaseibacillus rhamnosus GG strain:ATCC 53103

Project description:Lacticaseibacillus rhamnosus GG strain:ATCC 53103 Genome sequencing

| PRJNA185406 | ENA

Ribosome profiling and RNA sequencing reveal genome-wide cellular translation and transcription regulation under osmotic stress in Lactobacillus rhamnosus ATCC 53103

Project description:To determine whether osmotic pressure affects the translation efficiency of Lactobacillus rhamnosus, the ribosome profiling assay was performed to analyze the changes in translation efficiency in L. rhamnosus ATCC 53103. Under osmotic stress, differentially expressed genes (DEGs) involved in fatty acid biosynthesis and metabolism, ribosome, and purine metabolism pathways were co-regulated with consistent expression direction at translation and transcription levels. DEGs involved in the biosynthesis of phenylalanine, tyrosine, and tryptophan, and the phosphotransferase system pathways also were co-regulated at translation and transcription levels, while they showed opposite expression direction at two levels. Moreover, DEGs involved in the two-component system, amino acid metabolism, and pyruvate metabolism pathways were only regulated at the transcription level. And DEGs involved in fructose and mannose metabolism were only regulated at the translation level. The translation efficiency of DEGs involved in the biosynthesis of amino acids was downregulated while in quorum sensing and PTS pathways was upregulated. In addition, the ribosome footprints accumulated in open reading frame regions resulted in impaired translation initiation and elongation under osmotic stress. In summary, L. rhamnosus ATCC 53103 could respond to osmotic stress by translation regulation and control the balance between survival and growth of cells by transcription and translation.

2021-12-15 | GSE188929 | GEO

Synbiotic impact of tagatose on viability of Lactobacillus rhamnosus strain GG mediated by the phosphotransferase system (PTS)

Project description:The presence of tagatose in Lactobacillus rhamnosus strain GG caused induction of a large number of genes associated with carbohydrate metabolism including the phosphotransferase system. In addition, these results indicate the tagatose enhanced the growth of Lactobacillus casei 01 and Lactobacillus rhamnosus strain GG and their probiotic activities by activating tagatose-associated PTS networks. Two-condition experiment, Lactobacillus rhamnosus GG with glucose vs. Lactobacillus rhamnosus GG with tagatose. For preparing the total RNA, Lactobacillus rhamnosus GG cells were grown at 37M-BM-0C in prebiotic minimum medium supplemented with 2% glucose or tagatose for 24 h.

2013-09-19 | E-GEOD-41073 | biostudies-arrayexpress

Bile stress response in probiotic Lactobacillus rhamnosus GG

Project description:Transcriptional profiling of probiotic Lactobacillus rhamnosus strain GG mid-exponential pH-controlled bioreactor cultures before and after exposure to bovine bile (0.2% ox gall). Keywords: bile, stress response Cell samples from four biological replicates were harvested right before (time point 0 min) and 10, 30 and 120 min after bile treatment. Each sample was compared to a common reference sample (time point 0 min, mid-exponential growth phase Lactobacillus rhamnosus GG cultures). A total of 12 hybridizations were performed using balanced dye-swap design. Dyes were balanced between compared sample pairs and between biological replicates.

2011-01-01 | E-GEOD-22536 | biostudies-arrayexpress

Lacticaseibacillus rhamnosus GG strain:GG

Project description:Lactobacillus rhamnosus GG derivative genome sequencing

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Bile stress response in probiotic Lactobacillus rhamnosus GG

2011-01-01 | GSE22536 | GEO

Synbiotic impact of tagatose on viability of Lactobacillus rhamnosus strain GG mediated by the phosphotransferase system (PTS)

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Growth phase-associated changes in Lactobacillus rhamnosus GG

Project description:Transcriptional profiling of probiotic Lactobacillus rhamnosus GG during growth in industrial-type whey medium in pH-controlled bioreactor cultures at two different growth pH: 4.8 and 5.8. Keywords: growth phase, growth pH

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Lacticaseibacillus rhamnosus GG

Project description:Growth phase-associated changes in Lactobacillus rhamnosus GG

| PRJNA140663 | ENA

Surfacome analysis of Lactobacillus rhamnosus GG and Lactobacillus rhamnosus Lc705

Project description:The present study reports comparative surfacomics (study of cell-surface exposed proteins) of the probiotic Lactobacillus rhamnosus strain GG and the dairy strain Lc705.

2016-07-01 | PXD001201 | Pride

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