Project description:Clonorchiasis is associated with bile duct malignancy and the subsequent development of cholangiocarcinoma. Although this is likely caused by adult Clonorchis sinensis and its excretory-secretory products (ESP), the precise molecular mechanisms remain obscure. To evaluate the effect of C. sinensis infection on differential gene expression in host hepatocytes, we used cDNA microarrays in human cholangiocarcinoma cells through the mimicking of C. sinensis infestation, and analyzed differential mRNA expression patterns of host cells. Keywords: Time course
Project description:Analysis of host response to the infected Clonorchis sinensis metacercariae and adult worm. The infected tissues evidenced altered expression of genes involved in systems such as immune response and cell cycle regulation, as compared with normal tissues.
Project description:Analysis of host response to the infected Clonorchis sinensis metacercariae and adult worm. The infected tissues evidenced altered expression of genes involved in systems such as immune response and cell cycle regulation, as compared with normal tissues. Total RNA obtained from isolated liver tissues subjected to 1, 2, 4, and 6 weeks post-infection compared to uninfected liver tissues.
Project description:We examined gene expression profiles (27028 genes) in the livers of Sprague-Dawley rats with no infection and at 2 and 4 weeks after Clonorchis sinensis infection using Whole Rat Genome Microarray 4x44K v3 (GPL14745, Agilent-028282)
