Project description:The objective of this study was to decipher the metabolism expressed by Lactobacillus delbrueckii subsp. delbrueckii CIRM-BIA865 during soy juice fermentation using transcriptomics. The whole genome was sequenced, assembled and annotated. CIRM-BIA865 was then used to ferment soy juice to produce a soy-based yogurt. Samples were analysed in kinetics during fermentation, at pH values of 6.5, 6, 5 and 4.6. RNA from CIRM-BIA865 were extracted and sequenced using paired-end Illumina. Reads were mapped using Bowtie2 on previously obtained genome of CIRM-BIA865. No mismatch were allowed. Reads mapped on CDS were counted using htseqcount.List of differentially expressed (DE) genes between two successive sampling times (determined by pH) were generated using DEseq2 with a modified t-test and a p-value adjusted by Bonferoni inferior to 0.05. Fold changes expressed how many times genes were induced along the fermentations.
Project description:We identified the proteins synthesized by Lactobacillus delbrueckii ssp. bulgaricus strain LBB.B5 in laboratory culture medium (MRS) at 37 �C and in milk at 37 and 4 �C. Cell-associated proteins were measured by gel-free, shotgun proteomics using high-performance liquid chromatography coupled with tandem mass spectrophotometry. A total of 635 proteins were recovered from all cultures, and among which 72 proteins were unique to or significantly more abundant in L. bulgaricus after growth in milk compared to MRS at 37 �C.
Project description:Transcriptional profiling of Lactobacillus delbrueckii subsp. bulgaricus 2038 during the growth in casein proteins conditioned medium compared with the start control (cells treated in whey conditioned medium). Identifying the genes that are differentially expressed during the growth of Lb. bulgaricus 2038 in casein proteins condition provides a starting point for the investigation of metabolic mechanisms.
