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Ralstonia solanacearum

ABSTRACT: Genome sequences of Ralstonia solanacearum infecting ginger plants

PROVIDER: PRJDB10588 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	DRR420885_1.fastq.gz	Fastqsanger.gz
	DRR420885_2.fastq.gz	Fastqsanger.gz
	DRR420886_1.fastq.gz	Fastqsanger.gz
	DRR420886_2.fastq.gz	Fastqsanger.gz

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Arabidopsis Col-5 challenged with Ralstonia solanacearum BCCF401

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Expression data from Arabidopsis thaliana Col-0 challenged with Ralstonia solanacearum race 4 (roots)

Project description:Bacterial wilt caused by Ralstonia solanacearum is a serious seed/soil borne disease that causes severe yield and quality losses in many plants. In order to understand the change in genome expression of inculated plants, microarray analysis were performed. Twenty one days old roots of Arabidopsis Col-0 were inoculated with Ralstonia solanacearum race 4 @ 10^9 & 10^8 cfu/ml in different plants, distil water were mock inoculated, after five days plants were taken for RNA extraction and hybridization on Affymetrix microarrays. Plants were incubated in growth chamber for disease development, temperature and humidity were maintained as per plant requirement for both treated and control plants.

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Expression data from Arabidopsis thaliana Col-0 challenged with Ralstonia solanacearum race 4

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HrpB-mediated biological control of Ralstonia solanacearum in Arabidopsis thaliana

Project description:Microarray analysis was performed on the aerial parts of 5 plants collected 24 hours after water treatment or inoculation by the Ralstonia solanacearum hrpB strain (avirulent strain) and 5 days (25% of wilted leaves) after challenge inoculation with the GMI1000 virulent R. solanacearum strain. We compared trancriptome expression level of protected plants (hrpB prinoculation) versus non protected plants (water treatment), 24H after treatment and 5 days (25% of wilted leaves) after challenge inoculation with the GMI1000 virulent R. solanacearum strain

2011-06-01 | E-MTAB-542 | biostudies-arrayexpress

Expression analysis of Ralstonia solanacearum strain GMI1000 at 20°C and 28°C in rich medium (CPG) and in planta

Project description:Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain GMI1000 at 20°C and 28°C in culture and in planta. The tropical strain GMI1000 cannot wilt tomato plants at 20°C although it can cause full-blown disease at 28°C. A 16 array study using total RNA recovered from the following: 8 separate cultures of Ralstonia solanacearum strain GMI1000 grown in rich medium-CPG (4 grown at 20°C and 4 grown at 28°C) 8 separate in planta samples of Ralstonia solanacearum strain GMI1000 harvested from diseased tomato plants cv. BonnyBest (4 recovered from plants grown at 20°C and 4 from plants grown at 28°C) Each array (4-plex format) measures the expression level of 5061 genes from Ralstonia solanacearum strain GMI1000 with 2 to 6, 40-70 mer probes per gene, with two-fold technical redundancy. The arrays also contain probes for intergenic regions with no technical replicates.

2012-01-15 | E-GEOD-33657 | biostudies-arrayexpress

Expression analysis of Ralstonia solanacearum strain UW551 at 20°C and 28°C in rich medium (CPG) and in planta

Project description:Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain UW551 at 20°C and 28°C in culture and in planta. The temperatel strain UW551 can wilt and cause full-blown disease on tomato plants at 28°C as well as at 20°C. A 16 array study using total RNA recovered from the following: 8 separate cultures of Ralstonia solanacearum strain UW551 grown in rich medium-CPG (4 grown at 20°C and 4 grown at 28°C) 8 separate in planta samples of Ralstonia solanacearum strain UW551 harvested from diseased tomato plants cv. BonnyBest (4 recovered from plants grown at 20°C and 4 from plants grown at 28°C) Each array (4-plex format) measures the expression level of 4318 genes from Ralstonia solanacearum strain UW551 with 2 to 6, 40-70 mer probes per gene, with two-fold technical redundancy. The arrays also contain probes for intergenic regions with no technical replicates.

2012-01-15 | E-GEOD-33661 | biostudies-arrayexpress

Ralstonia solanacearum strain GMI1000: Methylation profile

Project description:The aim of the project is to decipher the role of DNA methylation in the plant pathogenic bacteria Ralstonia solanacearum during host adaptation. As a first step, we present here the DNA methylation profile of the GMI1000 reference strain.

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Arabidopsis Col-5 challenged with Ralstonia solanacearum BCCF401

Project description:Arabidopsis plants were challenged with Ralstonia solanacearum isolate BCCF401 and expression profiles investigated during early and late wilt symptom development. Keywords: Disease state analysis A direct comparison was performed. Two replicate biological experiments were performed and two technical replicates with dye swap were included in each biological replicate.

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