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Oryza sativa Japonica Group strain:Nipponbare

ABSTRACT: Transcriptome of polyspermic rice zygotes produced by in vitro fertilization

PROVIDER: PRJDB10883 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR258364_1.fastq.gz	Fastqsanger.gz
	DRR258364_2.fastq.gz	Fastqsanger.gz
	DRR258365_1.fastq.gz	Fastqsanger.gz
	DRR258365_2.fastq.gz	Fastqsanger.gz
	DRR258366_1.fastq.gz	Fastqsanger.gz

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Project description:Phosphate starvation/sufficient rice seedling, root or shoot Pi-starvation or Pi-sufficient stresses responsible rice genes, including previously unannotated genes were identified by Illumina mRNA-seq technology. 53 million reads from Pi-starvation or Pi-sufficient root or shoot tissues were uniquely mapped to the rice genome, and these included 40574 RAP3 transcripts in root and 39748 RAP3 transcripts in shoot. We compared our mRNA-seq expression data with that from Rice 44K oligomicroarray, and about 95.5% (root) and 95.4% (shoot) transcripts supported by the array were confirmed expression both by the array and by mRNA-seq, Moreover, 11888 (root) and 11098 (shoot) RAP genes which were not supported by array, were evidenced expression with mRNA-seq. Furthermore, we discovered 8590 (root) and 8193 (shoot) previously unannotated transcripts upon Pi-starvation and/or Pi-sufficient.

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