Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens strain:HEK293T, HeLA, PC9

ABSTRACT: Amplicon sequencing of genome-edited cells established by single-cell dispensing.

PROVIDER: PRJDB13164 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR351109_1.fastq.gz	Fastqsanger.gz
	DRR351109_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Homo sapiens strain:HEK293T, HeLa, PC9

Project description:CGH array for human cell lines

| PRJDB14627 | ENA

Targeted sequencing of Cerberus T-cells (Cb-STs) and pentavalent specific T-cells (Penta-STs) targeting CMV, EBV, BKV, adenovirus and Aspergillus fumigatus

Project description:Amplicon-based sequencing of Pentavalent specific T-cells (unedited pathogen-specific T-cells targeting CMV, EBV, BKV, adenovirus and Aspergillus fumigatus) and Cerberus T-cells (CRISPR/Cas9 edited Glucocorticoid-resistant T-cells targeting CMV, EBV, BKV, adenovirus and Aspergillus fumigatus) for off-target sites prediction.

2021-01-14 | E-MTAB-9968 | biostudies-arrayexpress

Gene expression profiling of erlotinib-resistant PC9 cells.

Project description:The non-small cell lung carcinoma (NSCLC) PC9 cell line is an established preclinical model for tyrosine kinase inhibitors. To better understand gene expression changes in cells that survived the inhibitor treatment, we treated the EGFR-mutant PC9 cells with erlotinib, isolated RNA, and performed RNA-seq analysis. We were able to identify genes that are differentially expressed in erlotinib-treated cells compared to untreated. The results of this study will be integrated with single cell RNA-seq to address the utility of bulk RNA versus single cell RNA strategies in identifying biomarkers of drug resistance.

2020-12-19 | GSE148465 | GEO

GNPS Trypanosoma cruzi infection in HeLa cells

Project description:Single-probe single cell mass spectrometry (SCMS) analysis contains 3 sets of experiments: 1. SCMS analysis of 4 groups of cells: infected, bystanders, stained and control cells 2. SCMS analysis of 3 groups of cells (2 replicates): infected, correctly classified bystander, misclassified bystander cells. 3. SCMS-MS of 5 glycerophosphocholines at m/z 768.583, 780.5460, 782.5630, 808.5770 and 810.5940 that were acquired from individual HeLa cells.

2022-05-20 | MSV000089503 | GNPS

Effects of IL2RA non-coding and coding sequence edits on IL2RA expression in primary human T cells.

Project description:We edited an intronic IL2RA enhancer or exon in primary human naïve T cells. Electroporated cells were activated and sorted on IL2RA expression over three days. Amplicon sequencing was used to assess the effects on sequence edits on IL2RA expression.

2017-07-30 | GSE88960 | GEO

Homo sapiens strain:22Rv1

Project description:Rfx6 amplicon sequencing from genome edited 22Rv1 isogenic cell lines

| PRJNA289208 | ENA

Detect-seq on transfected HEK293T cells [Detect-seq data]

Project description:We used a new technology, named Detect-seq, to perform genome sequencing on transfected HEK293T cells to see DdCBEs' off-target mutations. Besides, targeted-amplicon sequencing and ATAC-seq data were applied to validate the results of Detect-seq.

2022-04-22 | GSE173859 | GEO

Detect-seq on transfected HEK293T cells [Targeted deep sequencing data]

2022-06-01 | GSE176089 | GEO

Tabula Sapiens

Project description:Tabula Sapiens is a single cell transcriptomic atlas of 24 human tissues and organs.

2022-05-13 | GSE201333 | GEO

Transcriptome analysis of PC9 cells with gefitinib or/and hypoxia treatment and comparison with gefitinib resistant PC9 cells and ALDH positive PC9 cells

Project description:Inevitable gefitinib resistance and relapse of the disease was the biggest hurdle to NSCLC treatment. Importantly, the role of hypoxia in solid tumor tissues in vivo in gefitinib acquired resistance and its relationship to lung cancer stem cells (LCSCs) has not been fully elucidated. Here, the PC9 cells were treated with short term gefitinib or/and hypoxia, also, PC9 gefitnib resistant (PC9-GR) cell line was established and ALDH positive PC9 cells were sorted by FACs. Transcriptome analysis among those PC9 cell groups revealed the important role of hypoxia in gefitinib acquired resistance and signaling transduction change, which may critical for NSCLC disease progression and recurrence.

2017-10-31 | GSE69599 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data