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Brevibacterium epidermidis NBRC 14811

ABSTRACT: Brevibacterium epidermidis NBRC 14811 genome sequencing project

PROVIDER: PRJDB1335 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	BCSJ01.dat.gz	Other
	BCSJ01.fasta.gz	Fasta.gz
	BCSJ01.master.dat	Other
	BCSJ01.dat.gz	Other

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Brevibacterium epidermidis

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Expression analysis of Gluconacetobacter xylinus NBRC 3288 delta-fnrG mutant

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Effect of Calcium Chloride Concentrations on the Transcriptome of Brevibacterium lines

Project description:A urease positive marine actinobacterium Brevibacterium lines was demonstrated to form and dissolve calcite precipitation in conditions with different concentration of Ca2+. Next-generation sequencing (NGS) was used to analyze the transcriptome of B. lines under 0, 50 and 150 mM Ca2+ after 24 h incubation to discover the differentially expressed genes involved. Results provide insight into the molecular response of B. lines stressed with different concentration of Ca2+.

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Transcriptome analysis of Bacillus subtilis NBRC 16449 grown on surface of boiled soybeans under the similar condition to production of Japanese traditional soybean-fermented food "natto"

Project description:Purpose:The goals of this study are to clarify the B. subtilis NBRC 16449 response to soybeans. Methods: B. subtilis NBRC 16449 cells were aerobically cultured in liquid LB, LB solidified with agar, or on surface of boiled soybeans to logarithmic growth phase. Total RNAs were extracted from bacterial cells by Hot-Phenol method. Samples for RNA-seq were prepared according to Illmina protocol available from the manufacture. The sequence reads that passed quality filters were analyzed at the transcript isoform level with bowtie v0.11.2. Results: Using an optimized data analysis workflow, we mapped around 15 million sequence reads per sample to the whole genome of B. subtilis BEST195 and identified 4271 transcripts in B. subtilis NBRC 16449 with Bowtie aligner. Read count per genome was extracted from known gene annotations with HTSeq program. Compared the transcriptomes of B. subtilis NBRC 16449 grown on LB solidified with agar to that grown on surface of boiled soybeans, about 5% of genes showed the different expression levels.

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Brevibacterium casei

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Staphylococcus epidermidis FRI909

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AI-2-dependent gene regulation in Staphylococcus epidermidis

Project description:Autoinducer 2 (AI-2), a widespread by-product of the LuxS-catalyzed S-ribosylhomocysteine cleavage reaction in the activated methyl cycle, has been suggested to serve as an intra- and interspecies signaling molecule, but in many bacteria AI-2 control of gene expression is not completely understood. Particularly, we have a lack of knowledge about AI-2 signaling in the important human pathogens Staphylococcus aureus and S. epidermidis. Here, to determine the role of LuxS and AI-2 in S. epidermidis, we analyzed genome-wide changes in gene expression in an S. epidermidis luxS mutant and after addition of AI-2 synthesized by over-expressed S. epidermidis Pfs and LuxS enzymes. Genes under AI-2 control included mostly genes involved in sugar, nucleotide, amino acid, and nitrogen metabolism, but also virulence-associated genes coding for lipase and bacterial apoptosis proteins. In addition, we demonstrate by liquid chromatography/mass-spectrometry of culture filtrates that the pro-inflammatory phenol-soluble modulin (PSM) peptides, key virulence factors of S. epidermidis, are under luxS/AI-2 control. Our results provide a detailed molecular basis for the role of LuxS in S. epidermidis virulence and suggest a signaling function for AI-2 in this bacterium. Keywords: wild type without glucose control vs luxS mutant vs luxS mutant with auto-inducer II

2008-10-10 | GSE9427 | GEO

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