Project description:Brevibacterium spp. are aerobic, nonbranched, asporogenous, gram-positive, rod-shaped bacteria which may exhibit a rod-coccus cycle when cells get older and can be found in various environments. Several Brevibacterium species have industrial importance and are capable of biotransformation of various contaminants. Here we describe the draft genome sequence of Brevibacterium epidermidis EZ-K02 isolated from nitrocellulose-contaminated wastewater environments. The genome comprises 3,885,924 bp, with a G + C content of 64.2%. This whole genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession PDHL00000000.
Project description:<p>To elucidate the metabolic response of Brevibacterium epidermidis TRM83610 to NaCl stress and facilitate its industrial application, this study employed metabolomics techniques to analyze changes in intracellular metabolites, particularly compatible solutes, under NaCl concentrations of 0, 5%, 10%, and 15%. The ectoine production capacity was further evaluated and optimized using response surface methodology. Results indicated significant metabolic differences among sample groups, with detection of various secondary metabolites associated with antimicrobial activity and plant growth promotion. Six compatible solutes dominated by ectoine were identified. The metabolic response strategies to NaCl stress included osmostress resistance, oxidative stress resistance, and survival competition strategies. Through response surface optimization, ectoine yield reached 440.60 mg/L, representing a 6.22-fold increase over the initial yield of 70.75 mg/L, demonstrating substantial application potential for this strain. This research enriches our understanding of the metabolic profile of B. epidermidis TRM83610, preliminarily reveals its metabolic responses to NaCl stress, and provides a foundational basis for its further development and utilization.</p>
Project description:<p>To elucidate the metabolic response of Brevibacterium epidermidis TRM83610 to NaCl stress and facilitate its industrial application, this study employed metabolomics techniques to analyze changes in intracellular metabolites, particularly compatible solutes, under NaCl concentrations of 0, 5%, 10%, and 15%. The ectoine production capacity was further evaluated and optimized using response surface methodology. Results indicated significant metabolic differences among sample groups, with detection of various secondary metabolites associated with antimicrobial activity and plant growth promotion. Six compatible solutes dominated by ectoine were identified. The metabolic response strategies to NaCl stress included osmostress resistance, oxidative stress resistance, and survival competition strategies. Through response surface optimization, ectoine yield reached 440.60 mg/L, representing a 6.22-fold increase over the initial yield of 70.75 mg/L, demonstrating substantial application potential for this strain. This research enriches our understanding of the metabolic profile of B. epidermidis TRM83610, preliminarily reveals its metabolic responses to NaCl stress, and provides a foundational basis for its further development and utilization.</p>
Project description:The custom-made S. epidermidis GeneChips(Shanghai Biochip Co., Ltd) included qualifiers representing open reading frame (ORF) sequences identified in the genomes of the S. epidermidis strain RP62A, as well as unique ORFs in S. epidermidis strain 12228. The GeneChips were composed of cDNA array containing PCR products of 2316 genes and oligonucleotide array containing 252 genes.Two-component regulatory systems (TCSs) play a pivotal role in bacterial adaptation, survival, and virulence by sensing changes in the external environment and modulating gene expression in response to a variety of stimuli.To investigate the regulatory role of LytSR, one of the TCSs identified in the genomes of S. epidermidis, we used the GeneChips to perform a transcriptional profile analysis of the wild strain and lytSR mutant.