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Drosophila sechellia strain:K-S10

ABSTRACT: Differentially-expressed genes between Drosophila sechellia female adult flies that are fed with control (normal) diets and high-sugar diets

PROVIDER: PRJDB13401 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR359638.fastq.gz	Fastqsanger.gz
	DRR359639.fastq.gz	Fastqsanger.gz
	DRR359640.fastq.gz	Fastqsanger.gz
	DRR359641.fastq.gz	Fastqsanger.gz
	DRR359642.fastq.gz	Fastqsanger.gz

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Drosophila melanogaster strain:Oregon R

Project description:Differentially-expressed genes between Drosophila melanogaster female adult flies that are fed with control (normal) diets and high-sugar diets

| PRJDB13402 | ENA

Genomics analysis of Drosophila sechellia response to Morinda citrifolia fruit diet

Project description:Drosophila sechellia is an island endemic host specialist that has evolved to consume the toxic fruit of Morinda citrifolia, also known as noni fruit. Recent studies by our group and others have examined genome-wide gene expression responses of fruit flies to individual highly abundant compounds found in noni responsible for the fruit’s unique chemistry and toxicity. In order to relate these reductionist experiments to the gene expression responses to feeding on noni fruit itself, we fed rotten noni fruit to adult female D. sechellia and performed RNA-sequencing. Combining the reductionist and more wholistic approaches, we have identified candidate genes that may contribute to each individual compound and those that play a more general role in response to the fruit as a whole. Using the compound specific and general responses, we used transcription factor prediction analyses to identify the regulatory networks and specific regulators involved in the responses to each compound and the fruit itself. The identified genes and regulators represent the possible genetic mechanisms and biochemical pathways that contribute to toxin resistance and noni specialization in D. sechellia.

2022-06-08 | GSE205467 | GEO

Transcriptional profiling of two drosophila species: Drosophila melanogaster versus Drophila sechellia

Project description:Transcriptional profiling of 3 day old virgin male and female adults comparing control male Drosophila melanogaster (MDM) versus male D sechellia (MDS) and comparing control female Drosophila melanogaster (FDM) versus female D sechellia (FDS). Goal was to determine why D sechellia is tolerant to octanoïc acid, the major toxic compound of Morinda citrifolia fruit

2018-01-15 | GSE52191 | GEO

Expression data from 0.15M and 0.7M-fed wild-type and ChREBP mutant, third instar Drosophila larval fat bodies (FBs)

Project description:Chronic high sugar feeding induces obesity, hyperglycemia, and insulin resistance in flies and mammals. These phenotypes are controlled by the fat body, a liver- and adipose- like tissue in Drosophila flies. To gain insight into the mechanisms underlying the connection between diet and insulin sensitivity, we used Illumina RNA-seq to profile gene expression in fat bodies isolated from chronically high sugar fed, wandering (post-prandial) third instar wild type larvae w(L3). These data were compared to control-fed wild-type wL3 fat bodies as well as those expressing transgenic interfering RNA (i) targeting CG18362 (Mio/dChREBP) in the fat body on both diets. Female VDRC w1118, cgGAL4, UAS-Dcr2 or UAS-ChREBPi(52606), cgGAL4, UAS-Dcr2 wandering third instar larvae were fed control (0.15M) or high (0.7M) sucrose and fat bodies isolated for RNA extraction.

2013-01-25 | E-GEOD-43734 | biostudies-arrayexpress

Transcriptomic analysis of octanoic acid response in Drosophila sechellia using RNA-sequencing

Project description:The dietary specialist fruit fly Drosophila sechellia has evolved to specialize on the toxic fruit of its host plant Morinda citrifolia. Toxicity of Morinda fruit is primarily due to high levels of octanoic acid (OA). Using RNA interference (RNAi), prior work found that knockdown of Osiris family genes Osiris 6 (Osi6), Osi7, and Osi8 led to increased susceptibility to OA in adult D. melanogaster flies, likely representing genes underlying a Quantitative Trait Locus (QTL) for OA resistance in D. sechellia. While genes in this major effect locus are beginning to be revealed, prior work has shown at least five regions of the genome contribute to OA resistance. Here, we identify new candidate OA resistance genes by performing differential gene expression analysis using RNA sequencing (RNA-seq) on control and OA-exposed D. sechellia flies. We found 104 significantly differentially expressed genes with annotated orthologs in D. melanogaster, including six Osiris gene family members, consistent with previous functional studies and gene expression analyses. Gene ontology (GO) term enrichment showed significant enrichment for cuticle development in upregulated genes and significant enrichment of immune and defense responses in downregulated genes suggesting important aspects of the physiology of D. sechellia that may play a role in OA resistance. In addition, we identified 5 candidate OA resistance genes that potentially underlie QTL peaks outside of the major effect region, representing promising new candidate genes for future functional studies.

2017-10-28 | GSE104422 | GEO

Expression data from control and high sugar-fed third instar Drosophila larvae

2011-06-21 | GSE30020 | GEO

Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO

Project description:Fruit flies were maintained either 40% sugar or 5% sugar diets for one week, then on 5% sugar for one further week. Whole flies were sampled at the end of weeks 1 and week 2. Persistent effects of past high sugar diets are identified.

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Expression data from 0.15M and 0.7M-fed wild-type and ChREBP mutant, third instar Drosophila larval fat bodies (FBs)

2013-01-25 | GSE43734 | GEO

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Project description:Chronic high sugar feeding induces obesity, hyperglycemia, and insulin resistance in flies and mammals. To gain insight into the mechanisms underlying this response, we profiled gene expression in chronically high sugar fed, wandering (post-prandial) third instar wild type larvae (L3). These data were compared to control-fed larvae as well as those (mid-L3) actively feeding for twelve hours on both diets. We used microarrays to detail the response of Drosophila larvae to high sugar-induced insulin resistance. Male Canton-S third instar larvae were fed control (0.15M) or high (1M) sucrose and selected for RNA extraction and hybridization on Affymetrix microarrays. Wandering L3 were selected as those in the top half of the vial with partial blue guts to confirm that they had stopped eating the (blue) food. Mid-L3 were selected as L2, aged overnight until early L3, then transferred to fresh control or high sucrose food for 12 more hours before selection.

2011-06-21 | E-GEOD-30020 | biostudies-arrayexpress

Genomics analysis of L-DOPA exposure in Drosophila sechellia

Project description:The dietary specialist fruit fly Drosophila sechellia evolved to feed primarily on the toxic fruit of Morinda citrifolia. Seeking the basis of adaptations to the unique chemistry of its host plant, we profiled genome-wide gene expression response to L-DOPA because it is highly abundant in M. citrifolia, critical for reproductive success of D. sechellia, and may influence toxin resistance in this species. Here we used a combination of functional genetics and genomics to identify a new gene, Esterase 6 (Est6) that is important for D. sechellia ecological adaptation to this new niche.

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