Project description:To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity.
Project description:To identify genes of the guard cell transkriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. Ost1-2 and slac1-3 mutants were compared to their wildtype.
Project description:LC-MS/MS analysis of purified mitochondria, purified chloroplast, and total leaf tissue from three species of flowering plants (Arabidopsis thaliana, Silene conica, and Agrostemma githago)
Project description:au14-01_tn - Observation of the effects of the product Tn on the expression of genes of A. thaliana. - Observation of the effects of the product Tn on the expression of genes of Arabidopsis thaliana related to nutrition and development. - Each plant is sprayed with a product. 24h or 48h after treatment, a leaf is removed for RNA extraction and analysis.
Project description:Water use efficiency has long been considered as an important target for the breeding of improved plant performance under drought. Minimizing leaf transpirational water loss via reduction of stomatal water conductance plays a key contributory role in drought resistance. In this study, we employed both guard cell (GC) targeted and constitutive ectopic overexpression of the Target of Rapamycin (TOR) kinase, a master regulator of multiple signaling networks in transgenic Arabidopsis thaliana, to investigate the impact of these expressed AtTOR transgenes in response to drought and water use efficiency. We performed genome-wide transcriptome analysis employing RNA-seq on the three Arabidopsis genotypes grown on the three water treatments, and further analysis will be used to elucidate the potential mechanism(s) contributing to differences in leaf stomatal physiology between WT and transgenic lines.
