Project description:Investigation gene expression level changes in four different melon fruits at four different developmental satges Transcriptomic analysis of developing melon fruits from two climacteric (cvs. Védrantais and Dulce) and two non-climacteric (cv. Piel de sapo and accession PI 161375) at 15, 25, 35 Days After Pollination (DAP) and Harvest stage
Project description:Gray mold disease affects fruits, vegetables and ornemental plants around the world, causing considerable losses every year. Its causing agent, the fungus Botrytis cinerea, produces infection cushions (IC) that are complex appressorial structure dedicated to the penetration of the plant tissues. A microarray analysis identified 1,231 up-regulated genes in IC-enriched mycelium, among which several genes coding for putative secreted proteins. These data were supported by qPCR analysis in vitro and in planta. They highlight a secretion of proteins involved in virulence like proteases, plant cell wall degrading enzymes and necrosis inducers. To validate these transcriptomic data, a proteomic analysis of the IC exo-proteome is required.
Project description:Plant-based diets could be a key source of microRNAs in animals. Plant microRNAs are cross-kingdom gene expression regulators that could modulate mammalian gene expression, influencing their physiology. Therefore, it is important to identify the microRNA expression profile of plant foods in order to identify potential target genes and biological functions in the mammalian host. Next-generation sequencing was applied to identify microRNAs in RNA samples derived from nuts (walnut and almond), vegetables (spinach) and fruits (orange, apple, olive, pear, and tomato). Our data revealed that edible plant contain a large number and diverse type of microRNAs.
