Project description:The goal of this study was to assay the extent of variation in chromatin organization between 3 ant castes (major and minor female workers and males) in one colony of Camponotus floridanus carpenter ant using ChIPseq.
Project description:The goal of this study was to assay the extent of variation in chromatin organization between 3 ant castes (major and minor female workers and males) in one colony of Camponotus floridanus carpenter ant using ChIPseq. 45 samples total: 30 ChIP samples and 3 inputs for total histone H3, 7 histone H3 PTMs and RNA Pol II in major, minor, and male ants; CBP in major and minor ants; the major H3K27ac sample was replicated. 4 ChIP samples for H3 and H3K27ac in brains of majors and minors, and 2 inputs. 2 RNAseq samples for major and minor ants head+thorax; 4 RNAseq samples for brain (majors and minors with 2 replicates each).
Project description:Study of the protein composition of the trophallactic fluid of the carpenter ant Camponotus floridanus. Samples were analyzed from ants with different functions (Nurse, Forager) coming from colonies with different ages (mature, young) either kept in lab or grown under natural conditions.
Project description:Social insects frequently engage in oral fluid exchange – trophallaxis – between adults, and between adults and larvae. Although trophallaxis is normally considered a simple food-sharing mechanism, we hypothesized that endogenous components of this fluid might underlie a novel means of chemical communication that directly influences colony organization. Through protein and small-molecule mass spectrometry and RNA sequencing in the ant Camponotus floridanus, we found that trophallactic fluid contains a set of specific proteins, hydrocarbons, microRNAs, and Juvenile Hormone, an important insect growth regulator. Comparison of trophallactic fluid proteins across social insect species (ants Camponotus fellah and Solenopsis invictis, honeybees Apis mellifera) revealed that many are regulators of growth and development. These results raise the possibility that, in addition to its role in food transfer, trophallaxis is a mode of communication that enables communal control of colony phenotypes.
Project description:Socially exchanged fluids, such as seminal fluid and milk, are a direct means through which an organism can influence conspecifics. When orally feeding larval offspring via trophallaxis, workers of the carpenter ant Camponotus floridanus were recently shown to transfer Juvenile Hormone III (JH), a key developmental regulator, as well as paralogs of JH esterase (JHE), an enzyme that catalyzes degradation of JH. Here we combine proteomic, phylogenetic and selection analyses to investigate the evolution of this esterase subfamily. We show that members of the Camponotus JHE-like protein family have undergone multiple duplications, experienced positive selection, and changed tissue localization to become abundantly and selectively present in trophallactic fluid. The Camponotus trophallactic esterases have maintained their catalytic triads but contain a number of positively-selected amino acid changes distributed throughout the protein, which possibly reflect an adaptation to the highly acidic trophallactic fluid of formicine ants. To determine whether these esterases might regulate larval development, we fed workers with a JHE-specific pharmacological inhibitor to introduce it into the trophallactic network. This inhibitor increased the likelihood of pupation of the larvae reared by these workers, similar and complementary to supplementation with JH. Together, these findings suggest that JHE-like proteins have evolved new roles in the Camponotus genus in inter-individual regulation of larval development.