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Fraxinus excelsior

ABSTRACT: Transcriptome profiling of Fraxinus excelsior clones challenged by Emerald ash borer

PROVIDER: PRJDB15336 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR453857_1.fastq.gz	Fastqsanger.gz
	DRR453857_2.fastq.gz	Fastqsanger.gz
	DRR453858_1.fastq.gz	Fastqsanger.gz
	DRR453858_2.fastq.gz	Fastqsanger.gz
	DRR453859_1.fastq.gz	Fastqsanger.gz

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Metabolomic profiling of Fraxinus excelsior genotypes tolerant or susceptible to ash dieback disease reveals changes in iridoid glycosides

Project description:European common ash, Fraxinus excelsior L., is currently threatened by Ash dieback (ADB) caused by the fungus, Hymenoscyphus fraxineus. To detect and identify metabolites that may be products of pathways important in contributing to resistance against H. fraxineus we performed untargeted metabolomic profiling on leaves from selected F. excelsior individuals showing strong tolerance or susceptibility to H. fraxineus. We identified sets of "features" [small molecules] that enabled strong discrimination between tolerant or susceptible genotypes of F. excelsior. Strikingly, tolerant F. excelsior lines exhibited low levels of iridoid glycosides, known anti-feeding deterrents. As Europe is threatened by Emerald Ash Borer (Agrilus planipennis), an invasive wood boring beetle native to East Asia that has devastated North American ash, our study raises the question whether selection for resistance to H. fraxineus leads to ecological trade-offs that result in susceptibility to emerging pests such as emerald ash borer.

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Project description:Fraxinus excelsior Raw sequence reads

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MetaboLights MTBLS372 - GNPS Metabolomic profiling of Fraxinus excelsior genotypes tolerant or susceptible to ash dieback disease reveals changes in iridoid glycosides

Project description:This data set is downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS372 Abstract:European common ash, Fraxinus excelsior L., is currently threatened by Ash dieback (ADB) caused by the fungus, Hymenoscyphus fraxineus. To detect and identify metabolites that may be products of pathways important in contributing to resistance against H. fraxineus we performed untargeted metabolomic profiling on leaves from selected F. excelsior individuals showing strong tolerance or susceptibility to H. fraxineus. We identified sets of “features” [small molecules] that enabled strong discrimination between tolerant or susceptible genotypes of F. excelsior. Strikingly, tolerant F. excelsior lines exhibited low levels of iridoid glycosides, known anti-feeding deterrents. As Europe is threatened by Emerald Ash Borer (Agrilus planipennis), an invasive wood boring beetle native to East Asia that has devastated North American ash, our study raises the question whether selection for resistance to H. fraxineus leads to ecological trade-offs that result in susceptibility to emerging pests such as emerald ash borer.

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Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation

Project description:We performed RNA-seq and proteomics on naturally infested green ash (F. pennsylvanica) trees at low, medium and high levels of increasing emerald ash borer (A. planipennis) infestation. Our integrative analysis of the RNA-Seq and proteomics data identified 14 proteins and 4 transcripts that contribute most to the difference between highly infested and low infested trees.

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Project description:A de novo 454-Illumina whole genome sequence for a self-pollinated progeny of a Fraxinus excelsior tree growing in woodland in Gloucestershire, UK

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Project description:Transforming ash genomics: a pan-genome resource for improved understanding of ash dieback resistance.

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Project description:Fraxinus excelsior and Fraxinus mandshurica transcriptional response to infection by Hymenoscyphus fraxineus

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