Project description:To elucidate the modulatory participation of miRNAs in mollusk biomineralization, we have employed high-throughput sequencing to identify miRNAs of pearl oyster, Pinctada fucata. Our study focused on the miRNA expression profile of the mantle, an organ responsible for shell formation of the oyster. The pearl oysters were cultured in the tank with the maintaining conditions of temperature 19 ℃, PH 8.1 and salinity 33‰ in recirculating seawater.
Project description:We have got a yellow shell variety of Pinctada fucata martensii after years of artificial breeding. To identify differentially expressed genes between yellow shell and normal black shell pearl oysters, we performed label-free proteomic analyses by LC-MS using mantle edge tissues.
Project description:To elucidate how does the gene expression profiles of whole transcriptome of P.fucata response to seawater acidification and warming, we have employed microarray as a tool to identify gene responses in these stress.The pearl oysters were added into the tanks with the maintaining conditions of temperature 25 °C and 31 °C, acidity pH7.8 and pH7.5, salinity 33‰ in recirculating seawater. The temperatures and pH values in the studies were near future levels and predicted for 2100 and 2300.
