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Tilapia

ABSTRACT: A genome-wide scan for signatures of recent selection in tilapia

PROVIDER: PRJDB1657 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR084053_1.fastq.gz	Fastqsanger.gz
	DRR084053_2.fastq.gz	Fastqsanger.gz
	DRR084054_1.fastq.gz	Fastqsanger.gz
	DRR084054_2.fastq.gz	Fastqsanger.gz
	DRR084055_1.fastq.gz	Fastqsanger.gz

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Global DNA methylation changes in Nile tilapia gonad during high-temperature induced masculinization

Project description:In fish, the sex determining mechanisms can broadly be classified as genotypic (GSD), temperature-dependent (TSD), or genotypic plus temperature effects (GSD+TE). For the fish species with TSD or GSD+TE, extremely high or low temperature can affect its sex determination and differentiation. For long time, the underlying changes in DNA methylation that occur during high or low temperature induced sex reversal have not been fully clarified. In this study, we used Nile tilapia as a model to perform a genome-wide survey of differences in DNA methylation in female and male gonads between control and high temperature induced groups using methylated DNA immunoprecipitation (MeDIP). We identified the high temperature induction-related differentially methylated regions (DMRs), and performed functional enrichment analysis for genes exhibiting DMR. These identified differentially methylated genes were potentially involved in the connection between environmental temperature and sex reversal in Nile tilapia. In this study, four samples (control females, CF; control males, CM; induced females, IF; induced males, IM) were analyzed.

2015-08-27 | E-GEOD-72386 | biostudies-arrayexpress

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Global DNA methylation changes in Nile tilapia gonad during high-temperature induced masculinization

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Leptin stimulates cellular glycolysis through a STAT3 dependent mechanism in tilapia

Project description:Purpose: The goal of this study was to assess the differential gene expression between control and leptin treated pituitary rostral pars distalis' of the tilapia (Oreochromis mossambicus). Methods: Pituitary mRNA profiles from control (PBS) and leptin (100nM) treated tilapia (Oreochromis mossambicus) pituitary rostral pars disatalis were generated by deep sequencing using Illumina MiSeq. The sequence reads that passed quality filters were analyzed at the transcript abundance level using ANOVA (ANOVA) and BowTie2 followed by Cufflinks. Results: Differential gene expression identified 1995 genes that were differentially expressed in the pituitary rostral pars distalis treated with leptin compared to PBS treated controls. These genes were indicated to be functionally involved in numerous biological processes including glycolyiss. Conclusions: Our study represents the first detailed pituitary rostral pars distalis transcriptomes in the tilapia and identifies leptin as a major stimulator of glycolysis at the cellular level in teleost fishes.

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Exploring the mechanism of Streptococcus agalactiae infecting tilapia intestinal tissue based on transcriptomics

Project description:The aim of this study is to investigate the mechanism of Streptococcus agalactis invading the intestine of tilapia

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Major gene expression changes and epigenetic remodelling in Nile tilapia muscle after just one generation of domestication

Project description:Background: The historically recent domestication of fishes has been essential for mankind due to the overexploitation of natural stocks and the increasing protein demand to meet the needs of a growing human population. Selection for relevant traits, such as growth, during domestication is a complex process whose epigenetic basis is poorly understood. Results: We have determined changes that occur in the muscle transcriptome after a single generation of Nile tilapia (Oreochromis niloticus) domestication. There was a downregulation of 2015 genes in fish reared in captivity compared to their wild progenitors. In contrast, several myogenic and metabolic genes that can affect growth potential were upregulated. Methods: RNA was extracted and ribosomal RNA was removed using the Ribo-Zero gold rRNA removal kit. RNA libraries were prepared using the NEBNext Ultra II directional RNA library prep kit for Illumina. In total, we obtained 480 million 150 bp paired-end reads. Conclusion: Taken together, our data indicate that thousands of genes were differentially expressed within a single generation of fish domestication.

2020-02-21 | GSE135811 | GEO

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