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Homo sapiens

ABSTRACT: Transcriptome data of MCF7 cells expressing doxycycline-inducible BRCA1, FOXO1, and control shRNAs.

PROVIDER: PRJDB16591 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR502060_1.fastq.gz	Fastqsanger.gz
	DRR502060_2.fastq.gz	Fastqsanger.gz
	DRR502061_1.fastq.gz	Fastqsanger.gz
	DRR502061_2.fastq.gz	Fastqsanger.gz
	DRR502062_1.fastq.gz	Fastqsanger.gz

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Project description:VCaP cells expressing inducible shRNAs for either ERG or a non-targeting control were treated with Doxycycline for 1, 3, 7 and 10 days prior to collection This experiment is designed to see which genes and pathways are modulated by ERG knockdown VCaP cells stably expressing a Doxycycline (dox)-inducible control nontargeting shRNA (Pak4) or an ERG shRNA (2217) were exposed to 100ng/ml Dox for the noted days.

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Effect of CHD4 and EWS-FLI1 knockdown on gene expression of Ewing sarcoma cells.

Project description:We established Ewing sarcoma cells (SKNMC) stably expressing doxycycline-inducible shRNAs targeting the NuRD component CHD4 or the fusion transcription factor EWS-FLI1 to study their influence in gene expression regulation.

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Re-activation of EWS-FLI1 suppressed FOXO1 expression as a novel therapeutic strategy for EwingÂ´s sarcoma

Project description:Transient transfection of a Ewing's Sarcoma cell line expressing type I EWS-FLI1 fusion and doxycycline-inducible short hairpin RNA against EWS-FLI1 (A673sh) In total, 7 samples were analysed: empty vector control and two nuclear directed AKT- and CDK2- phosphorylation resistant FOXO1 versions as well as sh-scrambled and sh-FOXO1, either in the presence (w.o. Doxy.) or absence of EWS-FLI1 (+ Doxy.) each 2 replicates

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Effect of COPI depletion on senescence model systems

Project description:To examine effects of COPI depletion on senescence transcriptional signatures we generated cell lines (IMR90 or IMR90 ER:RAS cells) with inducible 2 COPI shRNAs or control shRNAs and induced to senesce with either treatment with Bleomycin or 4OHT treatment. Transcriptional analysis was performed 3 days after induction of doxycycline inducible harpins and 10 days after senescence induction.

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Project description:Stable derivatives of the Reh cell line carrying inducible shRNAs were generated by transducing with lentivirus harbouring the pTRIPZ vector and enabling doxycycline-inducible expression of shRNAs together with an RFP reporter. Two independent lines carrying different shRNAs targeting ETV6 or a vector control were generated. Cells were treated for 0 or 7 days with doxycycline, and harvested for bulk RNA-seq.

2022-04-10 | E-MTAB-10308 | biostudies-arrayexpress

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