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RNA-seq following shRNA-mediated knock-down of ETV6-RUNX1 in the B-ALL cell line Reh

ABSTRACT: Stable derivatives of the Reh cell line carrying inducible shRNAs were generated by transducing with lentivirus harbouring the pTRIPZ vector and enabling doxycycline-inducible expression of shRNAs together with an RFP reporter. Two independent lines carrying different shRNAs targeting ETV6 or a vector control were generated. Cells were treated for 0 or 7 days with doxycycline, and harvested for bulk RNA-seq.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Jason Wray

PROVIDER: E-MTAB-10308 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulome analysis in B-acute lymphoblastic leukemia exposes Core Binding Factor addiction as a therapeutic vulnerability.

Wray Jason P JP Deltcheva Elitza M EM Boiers Charlotta C Richardson Simon Е SЕ Chhetri Jyoti Bikram JB Brown John J Gagrica Sladjana S Guo Yanping Y Illendula Anuradha A Martens Joost H A JHA Stunnenberg Hendrik G HG Bushweller John H JH Nimmo Rachael R Enver Tariq T

Nature communications 20221121 1

The ETV6-RUNX1 onco-fusion arises in utero, initiating a clinically silent pre-leukemic state associated with the development of pediatric B-acute lymphoblastic leukemia (B-ALL). We characterize the ETV6-RUNX1 regulome by integrating chromatin immunoprecipitation- and RNA-sequencing and show that ETV6-RUNX1 functions primarily through competition for RUNX1 binding sites and transcriptional repression. In pre-leukemia, this results in ETV6-RUNX1 antagonization of cell cycle regulation by RUNX1 as ...[more]

PMID: 36411286

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