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Mus musculus

ABSTRACT: Time course H3K27me3 ChIP-sequence of Ras-induced NIH 3T3 cells

PROVIDER: PRJDB1915 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR013262_1.fastq.gz	Fastqsanger.gz
	DRR013262_2.fastq.gz	Fastqsanger.gz
	DRR013263_1.fastq.gz	Fastqsanger.gz
	DRR013263_2.fastq.gz	Fastqsanger.gz
	DRR013264_1.fastq.gz	Fastqsanger.gz

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Mus musculus

Project description:H3K27me3 ChIP-sequence of Ras-induced NIH 3T3 cells

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Project description:RNA-sequence of Ras-induced NIH 3T3 cells

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Differentiation of NIH-3T3 cells to adipocytes by PPARg or EBF1 over-expression.

Project description:NIH-3T3 cells transduced with either EBF1-, PPARg2- or empty vector were stimulated with hormones to initiate adipocyte differentiation. RNA extraction was done using TriZol at d0, d2, d4 and d10 after stimulation. Samples were handled according to standard affymetrix protocols. Keywords = Adipogenesis Keywords = adipocyte Keywords = early B-cell factor 1 (EBF1) Keywords = commitment Keywords = differentiation Keywords = NIH-3T3 Keywords = pparg Keywords: time-course

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Transcription profiling of human B-cells and mouse NIH-3T3 cells for analysis of RNA synthesis, abundance and decay

Project description:This SuperSeries is composed of the following subset Series:; GSE9973: Half-life determination for human B-cells (BL41); GSE9975: newly transcribed RNA (nt-RNA) for IFN alpha and gamma time course; GSE9977: Expression data from NIH-3T3 cells treated with mock, 100 U/ml IFN alpha or 100 U/ml gamma for 1or 3h; GSE10011: Expression data from NIH-3T3 cells used for half-life determination Experiment Overall Design: Refer to individual Series

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Mus musculus

Project description:ETV6-NTRK3 fusion oncoprotein transduces NIH 3T3 cells

| PRJNA103047 | ENA

newly transcribed RNA (nt-RNA) for IFN alpha and gamma time course

Project description:Expression data from NIH-3T3 cells treated with mock, 100 U/ml IFN alpha or 100 U/ml gamma for 1 or 3h on nt-RNA labeled for 30-60 min at different times of interferon treatment Differential gene expression caused by IFN alpha or gamma was analyzed in newly transcribed RNA (nt-RNA) of NIH-3T3 cells treated for 1 or 3 h. RNA was labeled for 30 to 60 min and separated from total cellular RNA (tc-RNA) following Trizol RNA preparation and thiol-specific biotinylation We used microarrays to analyze the effects of IFNalpha and gamma treatment in newly transcribed RNA (nt-RNA) Keywords: time course

2009-09-30 | GSE9975 | GEO

Mus musculus

Project description:Ribosome profiling of NIH-3T3 cells infected with MCMV

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Bidirectional Introgression between Mus musculus domesticus and Mus spretus.

Project description:Introgressed variants from other species can be an important source of genetic variation because they may arise rapidly, can include multiple mutations on a single haplotype, and have often been pretested by selection in the species of origin. Although introgressed alleles are generally deleterious, several studies have reported introgression as the source of adaptive alleles-including the rodenticide-resistant variant of Vkorc1 that introgressed from Mus spretus into European populations of Mus musculus domesticus. Here, we conducted bidirectional genome scans to characterize introgressed regions into one wild population of M. spretus from Spain and three wild populations of M. m. domesticus from France, Germany, and Iran. Despite the fact that these species show considerable intrinsic postzygotic reproductive isolation, introgression was observed in all individuals, including in the M. musculus reference genome (GRCm38). Mus spretus individuals had a greater proportion of introgression compared with M. m. domesticus, and within M. m. domesticus, the proportion of introgression decreased with geographic distance from the area of sympatry. Introgression was observed on all autosomes for both species, but not on the X-chromosome in M. m. domesticus, consistent with known X-linked hybrid sterility and inviability genes that have been mapped to the M. spretus X-chromosome. Tract lengths were generally short with a few outliers of up to 2.7 Mb. Interestingly, the longest introgressed tracts were in olfactory receptor regions, and introgressed tracts were significantly enriched for olfactory receptor genes in both species, suggesting that introgression may be a source of functional novelty even between species with high barriers to gene flow.

| S-EPMC8784167 | biostudies-literature

Expression data from NIH-3T3 cells treated with mock, 100 U/ml IFN alpha or 100 U/ml gamma for 1or 3h

Project description:Differential gene expression caused by 1h and 3h of IFN alpha or gamma treatment was analyzed in total cellular RNA of NIH-3T3 cells compared to mock We used microarrays to analyze the effects of 1 and 3h of IFNalpha and gamma treatment in total cellular RNA Keywords: time course in total cellular RNA (tc-RNA)

2009-09-30 | GSE9977 | GEO

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