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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human B-cells and mouse NIH-3T3 cells for analysis of RNA synthesis, abundance and decay

ABSTRACT: This SuperSeries is composed of the following subset Series:; GSE9973: Half-life determination for human B-cells (BL41); GSE9975: newly transcribed RNA (nt-RNA) for IFN alpha and gamma time course; GSE9977: Expression data from NIH-3T3 cells treated with mock, 100 U/ml IFN alpha or 100 U/ml gamma for 1or 3h; GSE10011: Expression data from NIH-3T3 cells used for half-life determination Experiment Overall Design: Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Lars Dölken

PROVIDER: E-GEOD-10026 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Conserved principles of mammalian transcriptional regulation revealed by RNA half-life.

Friedel Caroline C CC Dölken Lars L Ruzsics Zsolt Z Koszinowski Ulrich H UH Zimmer Ralf R

Nucleic acids research 20090626 17

RNA levels in a cell are regulated by the relative rates of RNA synthesis and decay. We recently developed a new approach for measuring both RNA synthesis and decay in a single experimental setting by biosynthetic labeling of newly transcribed RNA. Here, we show that this provides measurements of RNA half-lives from microarray data with a so far unreached accuracy. Based on such measurements of RNA half-lives for human B-cells and mouse fibroblasts, we identified conserved regulatory principles ...[more]

PMID: 19561200

Publication: 1/2

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Transcription profiling of mouse NIH-3T3 cells treated with interferon alpha or gamma

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