Sporothrix schenckii strain:NBRC32961
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ABSTRACT:
PROVIDER: PRJDB19887 | ENA |
REPOSITORIES: ENA
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Project description:Sporothrix schenckii Genome sequencing and assembly
| PRJNA432267 | ENA
Project description:Sporothrix schenckii Genome sequencing and assembly
| PRJNA1077318 | ENA
Project description:Sporotrichosis, an implantation mycosis caused by the dimorphic fungus Sporothrix schenckii, gained attention over the two last decades due to its broad geographic range and prevalence in tropical and subtropical areas. The dimorphic switching from hyphal form to the yeast form is essential for the pathogenesis of S. schenckii. During the mycelium-to-yeast transition, there are many changes in asexual development, yeast-phase cell formation, cell wall integrity, and melanin synthesis. However, the mechanisms of the signaling pathways associated with these process remain unclear. In this study, transcriptome sequencing of both the yeast stage and mycelium of S. schenckii was carried out. The results showed that 12217 genes, including genes involved in signal transduction and chitin synthesis, were expressed differentially between the two stages. According to these results, a map of the signaling pathways, related to the dimorphic switch, was drawn. Overall, the transcriptome data and analysis presented here lay the foundation for further research into the molecular mechanisms controlling the dimorphic switch of S. schenckii and support the development of anti-S. schenckii strategies targeting genes related to signaling pathways.
2019-06-27 | GSE133322 | GEO
Project description:Sporothrix schenckii strain:SsEM7 Genome sequencing and assembly
| PRJNA401003 | ENA
Project description:Sporothrix schenckii 1099-18 Genome sequencing and assembly
| PRJNA218070 | ENA
Project description:We report the global transcriptomic differences occurring between mold and yeast phases of S. schenckii including changes in gene expression profiles associated with these distinct cellular phenotypes. Moreover, we also propose a new genome annotation which reveals a more complex transcriptional architecture than previously assumed.
2020-02-25 | GSE145856 | GEO
Project description:Map of dimorphic switching-related signaling pathways in Sporothrix schenckii based on its transcriptome
| PRJNA551118 | ENA
Project description:The pathogenic dimorphic fungus Sporothrix schenckii is the agent responsible for sporotrichosis, an important fungal infection with a worldwide distribution. Little is known about the population structure of S. schenckii, although recent molecular and phenotypic data seem to demonstrate that different genetic lineages exist within this species. The aim of this study was to determine, by sequence analysis of three protein coding loci (chitin synthase, beta-tubulin, and calmodulin), whether this variability is due to species divergence or intraspecific diversity in S. schenckii. We included in the analysis 60 isolates (59 of clinical and 1 of environmental origin) of this species from a wide geographical range. DNA sequence data from the three nuclear regions were used in a phylogenetic analysis. The combined analysis of the three loci revealed the presence of three major clades, one grouping all of the European isolates, another with only Brazilian isolates, and the third with isolates from other South American countries and Africa. A total of 14 100% bootstrap-supported nodes were shown, 6 of them representing putative phylogenetic species. Our data also demonstrated that most of these species prevail in different geographical regions.
| S-EPMC1594699 | biostudies-literature
Project description:Sporotrichosis is a polymorphic disease caused by a complex of thermodimorphic fungi including S. brasiliensis, S. schenckii sensu stricto (s. str.), S. globosa and S. luriei. Humans and animals can acquire the disease through traumatic inoculation of propagules into the subcutaneous tissue. Despite the importance of sporotrichosis as a disease that can take epidemic proportions there are just a few studies dealing with genetic polymorphisms and genomic architecture of these pathogens. The main objective of this study was to investigate chromosomal polymorphisms and genomic organization among different isolates in the S. schenckii complex. We used pulsed field gel electrophoresis (PFGE) to separate chromosomal fragments of isolated DNA, followed by probe hybridization. Nine loci (β-tubulin, calmodulin, catalase, chitin synthase 1, Internal Transcribed Spacer, Pho85 cyclin-dependent kinase, protein kinase C Ss-2, G protein α subunit and topoisomerase II) were mapped onto chromosomal bands of Brazilian isolates of S. schenckii s. str. and S. brasiliensis. Our results revealed the presence of intra and interspecies polymorphisms in chromosome number and size. The gene hybridization analysis showed that closely related species in phylogenetic analysis had similar genetic organizations, mostly due to identification of synteny groups in chromosomal bands of similar sizes. Our results bring new insights into the genetic diversity and genome organization among pathogenic species in the Sporothrix schenckii complex.
| S-EPMC3900657 | biostudies-literature