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Soil metagenome

ABSTRACT: Diversity of ammonia oxidizing bacteria and archaea in sugar corn field soil

PROVIDER: PRJDB3028 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR022211.fastq.gz	Fastqsanger.gz
	DRR022212.fastq.gz	Fastqsanger.gz
	DRR022213.fastq.gz	Fastqsanger.gz
	DRR022214.fastq.gz	Fastqsanger.gz
	DRR022215.fastq.gz	Fastqsanger.gz

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Project description:This study evaluated the ammonium oxidizing communities (COA) associated with a potato crop (Solanum phureja) rhizosphere soil in the savannah of Bogotá (Colombia) by examining the presence and abundance of amoA enzyme genes and transcripts by qPCR and next-generation sequence analysis. amoA gene abundance could not be quantified by qPCR due to problems inherent in the primers; however, the melting curve analysis detected increased fluorescence for Bacterial communities but not for Archaeal communities. Transcriptome analysis by next-generation sequencing revealed that the majority of reads mapped to ammonium-oxidizing Archaea, suggesting that this activity is primarily governed by the microbial group of the Crenarchaeota phylum. In contrast,a lower number of reads mapped to ammonia-oxidizing bacteria.

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