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Mus musculus

ABSTRACT: CAGE-seq analysis of Fus-knocked down N2A cells

PROVIDER: PRJDB3084 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR022334.fastq.gz	Fastqsanger.gz
	DRR022335.fastq.gz	Fastqsanger.gz
	DRR022336.fastq.gz	Fastqsanger.gz

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Exon array analysis of N2A cells after knocking down FUS

Project description:To analyze the role of Fus, Ewsr1, and Taf15 in alternative RNA processing, we performed exon array analysis in N2A cells using exon arrays. N2A cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s instructions.

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Bidirectional Introgression between Mus musculus domesticus and Mus spretus.

Project description:Introgressed variants from other species can be an important source of genetic variation because they may arise rapidly, can include multiple mutations on a single haplotype, and have often been pretested by selection in the species of origin. Although introgressed alleles are generally deleterious, several studies have reported introgression as the source of adaptive alleles-including the rodenticide-resistant variant of Vkorc1 that introgressed from Mus spretus into European populations of Mus musculus domesticus. Here, we conducted bidirectional genome scans to characterize introgressed regions into one wild population of M. spretus from Spain and three wild populations of M. m. domesticus from France, Germany, and Iran. Despite the fact that these species show considerable intrinsic postzygotic reproductive isolation, introgression was observed in all individuals, including in the M. musculus reference genome (GRCm38). Mus spretus individuals had a greater proportion of introgression compared with M. m. domesticus, and within M. m. domesticus, the proportion of introgression decreased with geographic distance from the area of sympatry. Introgression was observed on all autosomes for both species, but not on the X-chromosome in M. m. domesticus, consistent with known X-linked hybrid sterility and inviability genes that have been mapped to the M. spretus X-chromosome. Tract lengths were generally short with a few outliers of up to 2.7 Mb. Interestingly, the longest introgressed tracts were in olfactory receptor regions, and introgressed tracts were significantly enriched for olfactory receptor genes in both species, suggesting that introgression may be a source of functional novelty even between species with high barriers to gene flow.

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FUS regulates the alternative splicing of cell proliferation genes related to atherosclerosis in human umbilical vein endothelial cells

Project description:FUS is an RNA binding protein that plays an important role in various cellular processes including RNA splicing, DNA repair and transcriptional regulation. However, the RNA binding capacity of FUS in atherosclerosis is unclear.we knocked down FUS with siRNA to further study the overall transcriptional level and select alternative splicing (AS) of FUS regulation in human umbilical vein endothelial cells (HUVECs) by RNA sequencing. The qRT-PCR strategy was used to validate FUS-modulated genes.Knockdown of FUS had significant effects on gene expression in HUVECs. Knock-down of FUS resulted in 200 differentially expressed genes (DEGs) that were highly related to apoptotic process, signal transduction, multicellular organism development, cell adhesion and regulation of transcription, DNA-templated pathways. Importantly, FUS extensively regulated 2870 alternative splicing events with a significant difference. Functional analysis of its-modulated alternative splicing genes revealed they were highly enriched in cell cycle, cell population proliferation and G2/M transition of mitotic cell cycle pathways. The results of qRT-PCR were consistent with the RNA-seq analysis.

2025-05-19 | GSE203426 | GEO

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