Project description:To analyze the role of Fus, Ewsr1, and Taf15 in alternative RNA processing, we performed exon array analysis in N2A cells using exon arrays. N2A cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s instructions.
Project description:We performed exon array analysis to define and compare the transcriptomic changes, in terms of splicing and gene expression profilings, occurring in human neuroblastoma cells upon knocking down TDP-43 and FUS RNA-binding proteins (RBP), with the final aim to unravel the biological role of these RBPs in neuronal cells.
