Project description:The white rot fungi Pleurotus ostreatus was studied for its potential as a biological pretreatment agent, i.e- its potential for decomposing lignocellulosic biomass. P. ostreatus PC9 was manipulate to either overexpress or eliminate (by gene replacement) the transcriptional regulator CRE1, known to act as a repressor in the process of carbon catabolite repression. The parental PC9 and the two transformants were grown on microcrystalline cellulose and wheat straw (3 replicates each) and the content of the resulted secretomes was analyzed by LC-MS/MS. An extensive range of carbohydrate active enzymes (CAZymes) were affected by the modification of cre1 expression levels. The three fungi revealed also differentiation towards the carbon source used in the growth media.
Project description:Pleurotus ostreatus, also known as the oyster mushroom, is an active lignin decomposer in the forests. The genomes of the monokaryotic strains PC15 and PC9 have been used to characterize the content and distribution of transposable elements. This study analyzes the impact of transposable element insertions on the global transcriptome of P. ostreatus. The transcriptome of PC15 and PC9 has been analyzed in exponential growth during submerged fermentation in malt-yeast extract-sucrose medium RNAseq of two P. ostreatus strains: PC15 and PC9
