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Marchantia polymorpha subsp. ruderalis

ABSTRACT: Transcriptomic analyses on vegetative and reproductive tissues of wild type Marchantia polymorpha.

PROVIDER: PRJDB7023 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR130762_1.fastq.gz	Fastqsanger.gz
	DRR130762_2.fastq.gz	Fastqsanger.gz
	DRR130763_1.fastq.gz	Fastqsanger.gz
	DRR130763_2.fastq.gz	Fastqsanger.gz
	DRR130764_1.fastq.gz	Fastqsanger.gz

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Project description:In the present study, Marchantia polymorpha Mppcs loss of function mutants were generated through CRISPR/cas9 mediated genome-editing. To assess whether the knockout of MpPCS gene affects the transcription of M. polymorpha nuclear genes in unstressed condition, the Mppcs-2 knockout mutant and Cam2 wild-type transcriptomes were compared by RNA-Seq.

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Effect of PRC2 loss of function on gene and TE expression in Marchantia polymorpha

Project description:To evaluate role of PRC2 on transcriptional regulation in bryophytes, we generated loss of function mutants of a Ez homolog in Marchantia polymorpha by CRISPR/Cas9 based mutagenesis. To avoid lethality of PRC2 knockout, we first mutate MpKNOX2 gene then additionally knocked MpEz1 out. Then we performed comparative transcriptome analyses using data obtained from Mpknox2 ez1 and Mpknox2.

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