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Schizosaccharomyces pombe

ABSTRACT: ChIP-seq analysis of RNA polymerase II and histone H3 in fission yeast cells.

PROVIDER: PRJDB712 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR003329.fastq.gz	Fastqsanger.gz
	DRR003330.fastq.gz	Fastqsanger.gz
	DRR003331.fastq.gz	Fastqsanger.gz
	DRR003332.fastq.gz	Fastqsanger.gz
	DRR003333.fastq.gz	Fastqsanger.gz

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Project description:Acetylation and dimethylation of lysine 9 on histone H3 are prominent marks of euchromatin and heterochromatin, respectively. Moreover, histone acetylation has been linked to lipid metabolism. We have previously identified the transcription factor Cbf11 as a regulator of lipid metabolism and genome integrity in the fission yeast. Cut6, the acetyl-CoA carboxylase, is one of direct targets of Cbf11. To link the role of Cbf11 in lipid metabolism and chromatin regulation we have performed ChIP-seq of H3K9ac, H3K9me2 and H3 in WT and cbf11KO strains of Schizosaccharomyces pombe in three biological replicates. Strain genotypes: wild type JB32 (h+s); cbf11 knock-out MP44 (h+ cbf11::natR).

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