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Bombyx mori

ABSTRACT: Transcriptome of BmN4 cells infected with BmNPV T3 or La strains

PROVIDER: PRJDB8648 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR188192_1.fastq.gz	Fastqsanger.gz
	DRR188192_2.fastq.gz	Fastqsanger.gz
	DRR188193_1.fastq.gz	Fastqsanger.gz
	DRR188193_2.fastq.gz	Fastqsanger.gz
	DRR188194_1.fastq.gz	Fastqsanger.gz

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Project description:The molecular mechanism involved in BmNPV resistance was investigated using a genome wide microarray in the midgut tissues of BmNPV infected resistant (Sarupat) and susceptible (CSR-2) Indian silkworm races. In the resistant race, 735 genes were upregulated and 589 genes were down regulated at 12 hours of BmNPV post infection. Similarly in case of susceptible race, 2183 genes were up regulated and 2115 genes were down regulated. Among the differentially expressed genes, nine upregulated and eight down regulated genes were validated using real time qPCR analysis. In Sarupat, the significantly upregulated genes are vacuolar protein sorting associated gene, X fin like protein and Carboxy peptidase E like protein in BmNPV infected midguts, whereas the prominent down regulated genes are glutamate receptor ionotropic kainite 2-like, BTB/POZ domain and transferrin. In the case of CSR-2, the considerably upregulated genes are Peptidoglycan recognition protein S6 precursor and rapamycin while the conspicuous down regulated genes are facilitated trehalose transporter and zinc transporter ZIP1-like gene. Our results provided a vital insights of Bombyx mori in reference with its molecular mechanism in immune response against the BmNPV invasion.

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Bombyx mori nucleopolyhedrovirus strain:T3

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Project description:RNAs associating with PIWI proteins were Immunoisolated from BmN4 cells. Sequence libraries were generated with NEBNext Small RNA Library Prep Set for Illumina(NEB). Libraries were sequenced using Illumina MiSeq (single-end, 51 cycles).

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